Biotinylated Human Glypican 3 / GPC3 Protein, His,Avitag™, premium grade
分子别名（Synonym）
GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1
表达区间及表达系统（Source）
Biotinylated Human Glypican 3, His,Avitag, premium grade (GP3-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).
Predicted N-terminus: Gln 25 & Ser 359
该产品在我们严格的质量控制体系下生产，其中包含包括无菌和内毒素检测在内的全方位测试。产品性能经过精心验证，并针对细胞培养用途及临床前研究阶段的其他应用进行相容性测试。当准备向后期临床阶段推进时，我们还提供定制化的GMP蛋白质服务，可根据您的需求量身定制。我们将与您合作，根据您的要求定制开发符合GMP级别的产品，同时满足基于细胞治疗的细胞制造中原料和辅助材料的使用要求。
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 26.4 kDa respectively. The protein migrates as 33 kDa, 43 kDa and 70-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method / rFC method.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介绍
磷脂酰肌醇蛋白聚糖-3（Glypican-3，GPC3）亦称肠道蛋白OCI-5、GTR2-2或MXR7，属于磷脂酰肌醇蛋白聚糖家族成员。该蛋白在肺、肝和肾脏中高表达，具有抑制DPP4二肽基肽酶活性的功能。GPC3可能参与中胚层来源为主的组织器官生长抑制/调节过程，并通过调控IGF2与其受体的相互作用进而影响其生物学功能。
关键字： Glypican 3;GPC3蛋白;GPC3重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group（股票代码：301080）是成立于2010年的跨国生物科技公司，是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球，横跨亚洲、北美洲、欧洲，在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家，与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。