Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag™ (MALS & SPR verified)
分子别名（Synonym）
FcRn,FCGRT & B2M
表达区间及表达系统（Source）
Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (FCM-H82W7) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1 (FCGRT) & P61769-1 (B2M)).
Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)
蛋白结构（Molecular Characterization）
Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.2 kDa (FCGRT) and 11.7 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 38-40 kDa (FCGRT) and 12 kDa (B2M) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.
背景介绍
FCGRT与B2M异源二聚体蛋白（FcRn复合物）由两个亚基组成：p51（相当于FCGRT）和p14（相当于β2-微球蛋白），形成I类MHC样异源二聚体结构。免疫球蛋白G Fc片段受体转运蛋白α亚基（FCGRT）可与γ型单体免疫球蛋白的Fc区结合，介导乳汁中免疫球蛋白G的吸收。FCGRT可能在母胎间免疫球蛋白G转运过程中发挥作用。β2-微球蛋白（B2M）是I类主要组织相容性复合体（MHC）的组成成分，参与向免疫系统呈递肽抗原。
关键字： FcRn (FCGRT & B2M);FCGRT蛋白;B2M重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group（股票代码：301080）是成立于2010年的跨国生物科技公司，是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球，横跨亚洲、北美洲、欧洲，在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家，与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。