产品概述
PRAS40 Rabbit Monoclonal Antibody
Rabbit Monoclonal antibody
Rabbit
WB,IP
Human,Rat
产品性能
Unconjugated
Unmodified
IgG
Monoclonal Antibody
Liquid
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Affinity Purification
免疫原
AKT1S1
AKT1S1; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate
84335
Q96B36
产品应用
WB: 1:500-1:1000 IP: 1:20
Calculated MW: 27 kDa; Observed MW: 40 kDa
研究背景
PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183.
研究领域
Cell Biology
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