产品概述
Crotonyl-Histone H2B (Lys12) Rabbit Monoclonal Antibody
Rabbit Monoclonal antibody
Rabbit
WB,IHC-F,IHC-P,ICC/IF
Human, Mouse, Rat
产品性能
Unconjugated
Crotonylated
IgG
Monoclonal Antibody
Liquid
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Affinity Purification
免疫原
H2BC4
H2BK11cr, Histone H2B.1 A;Histone H2B.a (H2B/a);Histone H2B.g (H2B/g);Histone H2B.h (H2B/h);Histone H2B.k (H2B/k);Histone H2B.l (H2B/l)
3018
P33778
产品应用
WB: 1:500-1:1000 IHC: 1:50-1:100 IF: 1:50-1:200
Calculated MW:14 kDa;Observed MW: 14 kDa
研究背景
Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H2B at Lys11 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.
研究领域
Epigenetics and Nuclear Signaling
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