产品概述
| 产品名称(Product Name) | MonoMethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody |
| 描述(Description) | Rabbit Monoclonal antibody |
| 宿主(Host) | Rabbit |
| 应用(Application) | WB,IHC-F,IHC-P,ICC/IF |
| 种属反应性(Reactivity) | Human, Mouse, Rat |
产品性能
| 偶联物(Conjugation) | Unconjugated |
| 修饰(Modification) | Monomethylated |
| 同种型(Isotype) | IgG |
| 克隆(Clonality) | Monoclonal Antibody |
| 形式(Form) | Liquid |
| 存放说明(Storage) | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 储存溶液(Buffer) | Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. |
| 纯化方式(Purification) | Affinity Purification |
免疫原
| 基因名(Gene Name) | H3C1 |
| 别名(Alternative Names) | H3K9me; Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f |
| 基因ID(Gene ID) | 8350 |
| 蛋白ID(SwissProt ID) | P68431 |
产品应用
| 稀释比(Dilution Ratio) | WB: 1:500-1:1000 IHC: 1:50-1:100 IF: 1:50-1:200 |
| 蛋白分子量(Molecular Weight) | Calculated MW:15 kDa;Observed MW: 17 kDa |
研究背景
Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, tri-methylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors.
研究领域
Epigenetics and Nuclear Signaling
关键字: H3C1;MonoMethyl-Histone;H3;(Lys9);Rabbit;Monoclonal;Antibody;一抗
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