产品概述
NEDD8 (5F5) Rabbit Monoclonal Antibody
Rabbit Monoclonal Antibody
Rabbit
WB,IHC-P,ICC/IF,FC,IP,IF-P
Human,Mouse,Rat
产品性能
Unconjugated
Unmodified
IgG
Monoclonal
Liquid
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
Affinity purification
免疫原
NEDD8
NEDD8; Nedd-8;
4738
Q15843
产品应用
WB 1:2000, IHC-P/IF-P 1:50, ICC/IF 1:50, FCM 1:50, IP 1:20
9kDa
研究背景
Neural precursor cell-expressed developmentally downregulated protein 8 (NEDD8), also known as Rub1 (related to ubiquitin 1) in plants and yeast, is a member of the ubiquitin-like protein family. The covalent attachment of NEDD8 to target proteins, termed neddylation, is a reversible, multi-step process analogous to ubiquitination. Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53 (PubMed:9694792, PubMed:10318914, PubMed:10597293, PubMed:11953428, PubMed:15242646, PubMed:14690597). Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins (PubMed:9694792, PubMed:10318914, PubMed:10597293, PubMed:11953428, PubMed:20688984). Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity (PubMed:15242646). Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M (PubMed:14690597).
研究领域
武汉恩玑生命科技有限公司
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