产品概述
| 产品名称(Product Name) | TF2B (18H9) Rabbit Monoclonal Antibody |
| 描述(Description) | Rabbit Monoclonal Antibody |
| 宿主(Host) | Rabbit |
| 应用(Application) | WB |
| 种属反应性(Reactivity) | Human,Mouse,Rat |
产品性能
| 偶联物(Conjugation) | Unconjugated |
| 修饰(Modification) | Unmodified |
| 同种型(Isotype) | IgG |
| 克隆(Clonality) | Monoclonal |
| 形式(Form) | Liquid |
| 存放说明(Storage) | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 储存溶液(Buffer) | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
| 纯化方式(Purification) | Affinity purification |
免疫原
| 基因名(Gene Name) | GTF2B |
| 别名(Alternative Names) | gtf2b; S300 II; TF IIB; TF2B; TFIIB; |
| 基因ID(Gene ID) | 2959 |
| 蛋白ID(SwissProt ID) | Q00403 |
产品应用
| 稀释比(Dilution Ratio) | WB 1:1000-1:5000 |
| 蛋白分子量(Molecular Weight) | 35kDa |
研究背景
General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP- independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841). Modulates transcription start site selection (PubMed:10318856). Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).
研究领域
关键字: GTF2B;TF2B;(18H9);Rabbit;Monoclonal;Antibody;一抗
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