Cynomolgus CD3 epsilon Protein, His Tag, low endotoxin (MALS verified)
分子别名（Synonym）
FLJ18683, T3E, TCRE, CD3E, CD3-epsilon
表达区间及表达系统（Source）
Cynomolgus CD3 epsilon, His Tag (CDE-C5226) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (Accession # Q95LI5-1).
Predicted N-terminus: Gln 22
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.1 kDa. The protein migrates as 18-23 kDa under reducing (R) condition, and 33-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介绍
CD3e分子（亦称CD3E）是一种T细胞表面单次跨膜的I型膜糖蛋白。该蛋白包含1个免疫球蛋白样结构域和1个ITAM结构域。CD3E与CD3-γ、CD3-δ、CD3-ζ链以及T细胞受体α/β和γ/δ异源二聚体共同构成T细胞受体-CD3复合物，该复合物在将抗原识别与多种细胞内信号转导通路耦合中起关键作用。编码ε、γ和δ多肽的基因均位于11号染色体的同一基因簇。其中ε多肽对T细胞发育至关重要，CD3E基因缺陷会导致严重免疫缺陷症。研究还表明CD3E基因与女性I型糖尿病易感性相关。目前已证实CD3E可与TOP2B、CD3EAP及NCK2发生相互作用。
关键字： CD3 epsilon;CD3 epsilon蛋白;CD3 epsilon重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group（股票代码：301080）是成立于2010年的跨国生物科技公司，是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球，横跨亚洲、北美洲、欧洲，在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家，与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。