Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (MALS verified)
分子别名（Synonym）
IL-17A, Interleukin-17A, CTLA-8, IL-17, IL-17F, Interleukin-17F, Cytokine ML-1, IL17A＆IL17F
表达区间及表达系统（Source）
Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (ILF-H52W6) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F) (Accession # Q16552-1 (IL-17A) & Q96PD4-1 (IL-17F)). Two sequential affinity purification steps were used to ensure exact 1:1 molar ratio of IL17A＆IL17F heterodimer.
Predicted N-terminus: Trp (IL-17A) & His (IL-17F)
蛋白结构（Molecular Characterization）
Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag, produced by co-expression of IL-17A and IL-17F, has a calculated MW of 18.5 kDa (IL-17A) and 16.8 kDa (IL-17F). Subunit IL-17A is fused with a Twin Strep tag at the N-terminus and subunit IL-17F is fused with a polyhistidine tag at the N-terminus. The protein migrates as 19-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介绍
白细胞介素17A（IL17A）亦称细胞毒性T淋巴细胞相关抗原8（CTLA8），是一种由活化T细胞产生的促炎细胞因子。该细胞因子可调控核因子κB（NF-κB）和丝裂原活化蛋白激酶的活性，同时能促进IL6和环氧化酶2（PTGS2/COX-2）的表达，并增强一氧化氮（NO）的生成。此外，研究发现IL17A存在糖基化和非糖基化两种形式。IL-17水平升高与类风湿关节炎、银屑病、多发性硬化等多种慢性炎症疾病密切相关。
关键字： IL-17A&IL-17F;IL-17A蛋白;IL-17F重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group（股票代码：301080）是成立于2010年的跨国生物科技公司，是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球，横跨亚洲、北美洲、欧洲，在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家，与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。