Human CD73 / NT5E Protein, His Tag (HPLC-verified) (active enzyme)
分子别名（Synonym）
CD73, NT5E, 5'-Nucleotidase, 5'-NT, NT5, NTE
表达区间及表达系统（Source）
Human CD73 Protein, His Tag (CD3-H52H7) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # P21589-1).
Predicted N-terminus: Trp 27
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 59.9 kDa. The protein migrates as 65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 24 months in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.
背景介绍
5‘-核苷酸酶（5’-NT）亦称外切-5‘-核苷酸酶或CD73，是由NT5E基因编码的一种酶。该酶主要催化腺苷一磷酸（AMP）向腺苷的转化。外切-5‘-核苷酸酶（5’-核糖核苷酸磷酸水解酶）在中性pH条件下可催化嘌呤5‘-单核苷酸向核苷的转化，其最适底物为AMP。细胞质和溶酶体中还存在其他形式的5‘-核苷酸酶，这些同工酶可通过底物亲和力、二价镁离子需求、ATP激活特性及无机磷酸抑制效应与外切-NT5相区分。罕见等位基因变异与成人型关节动脉钙化综合征（CALJA）相关，该病症累及髂动脉、股动脉及胫动脉，导致下肢及手足关节血液循环障碍并引发疼痛。
关键字： CD73;CD73蛋白;CD73重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group（股票代码：301080）是成立于2010年的跨国生物科技公司，是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球，横跨亚洲、北美洲、欧洲，在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家，与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。