SourceMouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P22646-1 (CD3E) & P11942-1 (CD3G)).Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 15.1 kDa (CD3E) and 15.2 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 19-22 kDa under reducing (R) condition, and 35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).
SDS-PAGE测定结果显示纯度>95%。
经 SEC-MALS 测定,大于 95%。
由 0.22 μm 过滤的 PBS 溶液(pH 7.4)冻干而成,以海藻糖为保护剂。
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