人血管生成素样蛋白7(ANGPTL7)ELISA试剂盒
货号:YB72149Hu
规格:48T/96T
种属:Human
范围:78.125-5000pg/ml
灵敏度:46.875pg/ml
用途:For quantitative detection of ANGPTL7 in serum, plasma, tissue homogenates andother biological fluids.
存储: 2-8°C for 6 months
注意:仅供研究使用。
组成成分
典型的数据和标准曲线
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. AntiANGPTL7 antibody was pre-coated onto 96-well plates. And the biotin conjugated antiANGPTL7 antibody was used as detection antibodies. The standards, test samples and biotinconjugated detection antibody were added to the wells subsequently, and washed with washbuffer. HRP-Streptavidin was added and unbound conjugates were washed away with washbuffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed byHRP to produce a blue color product that changed into yellow after adding acidic stop solution.The density of yellow is proportional to the ANGPTL7 amount of sample captured in plate. Readthe O.D. absorbance at 450nm in a microplate reader, and then the concentration of ANGPTL7can be calculated
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