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PKR 抗体
品牌:MedChemExpress (MCE)
货号:HY-P80283
存储条件:储存于 -20°C 下可保存 1 年。避免反复冻融。
运输条件:使用蓝冰运送。
产品活性:PKR Antibody 是一个非偶联、分子量约 62 kDa、兔源、抗 PKR 单克隆抗体。它可用于人背景下 WB、ICC/IF、IHC-P、IP 实验,且不带标记。
宿主:兔
反应物种:Human
推荐稀释比例:WB: 1:1,000-1:5,000 ;ICC/IF: 1:50-1:100 ;IHC-P: 1:50-1:100 ;IP: Use at an assay dependent concentration. Western blot analysis of extracts from THP-1 (lane 1) and Jurkat (lane 2) and Hela (lane 3) using PKR antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
标签:Free
克隆性:Recombinant, Monoclonal
偶联:Non-conjugated
修饰:Unmodified
亚细胞定位:Nucleus, Cytoplasm, perinuclear region.
研究领域:Signal Transduction
蛋白数据库:SwissProt: P19525 Human
同型:IgG
敏感性:Endogenous
详情参考:www.medchemexpress.cn/antibody/pkr-rabbit-mab.html