Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation.
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| Tested Applications | ELISA, FACS, IF, IHC-Fr, IHC-P, WB |
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| Dilution range | WB 1:500-1:2,000 IHC (F) 1:50-1:500 IHC (P) 1:50-1:500 IF 1:50-1:500 |
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| Reactivity | Other |
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| Application Notes | In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein) |
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| Antibody Type | Primary Antibody |
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| Host | Goat |
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| Clonality | Polyclonal |
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| Isotype | IgG |
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| Immunogen | Antigen: Purified recombinant peptide produced in E. coli.. Antigen Sequence: MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK |
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| Target | Red Fluorescent Protein |
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| Molecular Weight | 55 kDa |
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| Purity | This antibody is epitope-affinity purified from goat antiserum |
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| Purification | Epitope affinity purified |
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| Conjugation | Unconjugated |
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