化合物 C6 NBD Galactosylceramide|T85989|TargetMol

价格	￥4930
包装	100μg

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中文名称:化合物 C6 NBD Galactosylceramide	英文名称:C6 NBD Galactosylceramide
CAS:170212-26-7	品牌: TargetMol
产地: 美国	保存条件: Powder: -20°C for 3 years \| In solvent: -80°C for 1 year Shipping with blue ice.
产品类别: 抑制剂
货号: T85989

名称	C6 NBD Galactosylceramide
描述	C6 NBD galactosylceramide, an active derivative of galactosylceramide tagged with fluorescent nitrobenzoxadiazole (NBD), serves as a substrate for neutral β-glycosylceramidase (GCase) in studies of intracellular localization and metabolism of galactosylceramide (Ex=nm, Em=525) [1].
体外活性	C6-NBD-glucosylceramide (4 μM) undergoes transport to the Golgi apparatus in HT29 cells [1]. According to our recommended protocol, transcytosis of exogenous C6-NBD-GaICer follows these steps after endocytosis: Initially, C6-NBD-glucosylceramide is inserted at 10°C. Subsequently, cells are subjected to three cold HBSS rinses and maintained at 37°C in HBSS to facilitate endocytosis. After 10 minutes, residual probe on the cell surface is eliminated through either two (apical) or three (basal) 20-minute BSA washes at 10°C. Lipid analysis using one set of filters quantifies the endocytosis, while a second set incubated for either 0.5 or 1 hour at 37°C in HBSS + BSA measures the reemergence of intracellular C6-NBD-glucosylceramide on the cell surfaces. Post-incubation, a 10°C BSA wash is conducted before extracting the NBD lipids into chloroform/methanol from both apical and basal media, as well as the cells, which are then analyzed and quantitated by TLC.
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year Shipping with blue ice.

关键字： N-hexanoyl-NBD-Galactosylceramide|||NBD GalCer(d18:1/6:0)|||NBD Galactosylceramide (d18:1/6:0)|||C6-NBD-GalCer|TargetMol

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