Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Worthington: Types 1, 2, 3, and 4.
• Type 1 crude collagenase has the original balance of collagenase, caseinase, clostripain and tryptic activities.
• Type 2 contains higher relative levels of protease activity, particularly clostripain.
• Type 3 contains lowest levels of secondary proteases.
• Type 4 is designed to be especially low in tryptic activity to limit damage to membrane proteins and receptors.
• Type 5 contains higher collagenase and caseinase values.
• Type 6 contains high collagenase activity with a caseinase to collagenase ratio ~2:1. Designed to be enriched for Type II (col H) collagenase relative to Type I (col G).
• Type 7 contains collagenase and caseinase activities four-fold higher than collagenase Types 1 and 2.
关键字： 胶原酶;Collagenase;Worthington;LS004176;Collagenase, Type 2;