Description
The PM5100 3-color Pre-Stained Protein Ladder High Range is a ready-to-use three-color protein standard with 14 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5100 3-color Pre-Stained Protein Ladder High Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Features
Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
Three reference bands — 75 kDa (red), 40 kDa (green), and 20 kDa (blue)
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).
Quality Control
Under suggested conditions, PM5100 ExcelBand™ 3-color Pre-Stained Protein Ladder High Range resolves 14 major bands in SDS-PAGE (Tris-Glycine, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane.
Storage
4°C for 3 months
-20°C for long term storage
关键字： 预染蛋白Marker;PM5100;Smobio预染蛋白Marker;彩虹三色分子量：9-245 kDa;9-245 kDa;
【公司概况】北京鼎国生物成立于1997年，是国内成立最早的生物公司之一，公司拥有强大的营销团队，目前国内已有十七个分公司，在全国各地还有近三十多家长期业务合作伙伴。鼎国公司研发部设于北京市昌平区，面积2000多平方米，研发部拥有分子生物学、细胞生物学及免疫学实验所需大型仪器，能完成各类大型综合性实验服务。
【主营范围】鼎国生物的产品主要有试剂、试剂盒、耗材和仪器，涉及分子生物学、细胞生物学、免疫学、生物化学、微生物学、药物毒理学等。
【主营品牌】鼎国、Genview、德国Greiner耗材、Fisher耗材、Sigma-Vetec、SMBIO蛋白Marker、Scitecher胎牛血清、华美、Quawell分光光度计、东林仪器、博弗瑞德仪器等。
【企业理念】以人为本，追求个人与企业的共同发展。
【经营理念】诚信为本，质量至高，服务至上。
【管理理念】人文鼎国，文化鼎国，文明鼎国，建立高素质且有深厚凝聚力的团队。
广州鼎国生物技术有限公司成立于2001年，拥有一支专业、高效的销售和管理团队，经过十多年的发展，在广东市场获得良好的声誉。
【公司地址】广州市天河区东圃二马路61号东圃东湖工业区B栋2楼