Recombinant Human Her2/ERBB2 Protein (ECD, His Tag), HPLC‑verified｜Cat:10004‑H08HRecombinant Human Her2/ERBB2 Protein (ECD, His Tag), HPLC‑verified
Cat No: 10004‑H08H｜Official product page: https://www.sinobiological.com/recombinant‑proteins/human‑her2‑erbb2‑10004‑h08h
Specifications
Purity
≥ 90 % as determined by SDS‑PAGE. ≥ 90 % as determined by SEC‑HPLC. ≥ 90 % as determined by SEC‑MALS(Routinely tested).
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1‑Thr 652) of human ErbB2 (NP_004439.2) was fused with the polyhistidine tag at the C‑terminus.
Expression Host
HEK293 Cells
Predicted N Terminal
Thr 23
Molecule Mass
The recombinant human ErbB2 comprises 641 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, rh ErbB2 migrates as an approximately 100‑110 kDa protein in SDS‑PAGE under reducing conditions. The molecular weight of this protein is around 87.5 kDa verified by SEC‑MALS (Routinely tested).
Formulation
Lyophilized from sterile PBS, pH 7.4. Normally 5 % ‑ 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
Lyophilized powder shipped at ambient temperature; Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Stable for up to twelve months from date of receipt at ‑20℃ to ‑80℃. Aliquot protein, avoid repeated freeze‑thaw cycles.
Alternate Names
c‑ERB‑2 CD340 HER‑2/neu p185(erbB2)
Background
Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand‑bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase‑mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
Documents available on official page: Datasheet, COA, MSDS, Protocol, Reference citations.
关键字： HER-2;HER2;ERBB2;HER2蛋白;ERBB2蛋白;
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