Recombinant Human IL4 / Interleukin‑4 Protein
Cat No: 11846‑HNAE｜Official product page: https://cn.sinobiological.com/recombinant-proteins/human-il-4-11846-hnae
Specifications
Purity
≥ 95 % as determined by SDS‑PAGE. ≥ 95 % as determined by SEC‑HPLC. ≥ 95 % as determined by SEC‑MALS(Routinely tested).
Endotoxin
< 5 EU per mg of the protein.
Protein Construction
A DNA sequence encoding the mature form of human IL4 isoform 1 (P05112‑1) (His 25‑Ser 153) was expressed and purified with an initial Met.
Alternate Names
BCGF‑1 BCGF1 BSF‑1 BSF1 IL‑4 MGC79402
Background
Interleukin‑4, also known as IL4, is a secreted protein that belongs to the IL‑4 / IL‑13 family. Interleukin‑4 / IL4 has many biological roles, including the stimulation of activated B‑cell and T‑cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin‑4 / IL4 also regulates the expression of the low‑affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin‑4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B‑cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose‑dependent manner the IL4‑induced proliferation of lipopolysaccharide‑stimulated murine splenic B‑cells, of the murine T cell line CTLL‑2, and the murine pre‑B‑cell line BA/F3. QY also inhibited the IL4‑stimulated up‑regulation of CD23 expression by lipopolysaccharide‑stimulated murine splenic B‑cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4‑stimulated BA/F3 cells.
Documents available on official page: Datasheet, COA, MSDS, Protocol, Reference citations.
关键字： IL4;IL4细胞因子;白介素4;IL4蛋白;人白介素4;
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