Recombinant Apolipoprotein A4 (APOA4)
载脂蛋白A4(APOA4)重组蛋白
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Glu21~Asn235
Tags: N-terminal His and GST Tag
Subcellular Location: Secreted
Purity: > 97%
Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Original Concentration: 200µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.8
Predicted Molecular Mass: 42.8kDa
Accurate Molecular Mass: 55kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The[ IMPORTANT NOTE ]The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures. possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.
仅用于科研，不能用于临床诊断！所有产品仅供科研使用，不得用于人或动物的治疗等任何其他用途，不为任何个人提供产品和服务。
关键字： (APOA4);
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