Chemical Safety Data Sheet MSDS / SDS

LUCIFERASE

Revision Date:2026-03-21Revision Number:1

SECTION 1: Identification of the substance/mixture and of the company/undertaking

Product identifier

Product name: LUCIFERASE
CBnumber: CB3663963
CAS: 61970-00-1
EINECS Number: 263-359-4
Synonyms: EC 1.14.14.3;LUCIFERASE FIREFLY

Relevant identified uses of the substance or mixture and uses advised against

Relevant identified uses: For R&D use only. Not for medicinal, household or other use.
Uses advised against: none

Company Identification

Company: Chemicalbook
Address: Building 1, Huihuang International, Shangdi 10th Street, Haidian District, Beijing
Telephone: 010-86108875

SECTION 2: Hazards identification

GHS Label elements, including precautionary statements

Symbol(GHS)

Signal wordWarning

Precautionary statements

P260 Do not breathe dust/fume/gas/mist/vapours/spray.

P264 Wash skin thouroughly after handling.

P270 Do not eat, drink or smoke when using this product.

P301+P312 IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P314 Get medical advice/attention if you feel unwell.

P501 Dispose of contents/container to..…

Hazard statements

H302 Harmful if swallowed

H373 May cause damage to organs through prolonged or repeated exposure

SECTION 3: Composition/information on ingredients

Substance

Product name: LUCIFERASE
Synonyms: LUCIFERASE,Firefly Luciferase
CAS: 61970-00-1
EC number: 263-359-4

SECTION 4: First aid measures

General advice

First aiders need to protect themselves. Show this safety data sheet to the doctor in attendance.

If inhaled

After inhalation: fresh air. Call in physician.

In case of skin contact

In case of skin contact: Take off immediately all contaminated clothing. Rinse skin with water/ shower. Consult a physician.

In case of eye contact

After eye contact: rinse out with plenty of water. Call in ophthalmologist. Remove contact lenses.

If swallowed

After swallowing: immediately make victim drink water (two glasses at most). Consult a physician.

Most important symptoms and effects, both acute and delayed

The most important known symptoms and effects are described in the labelling (see section 2) and/or in section 11

Protection of first-aiders

For personal protection see section 8.

Notes to physician

No data available

SECTION 5: Firefighting measures

Suitable extinguishing media

Water Foam Carbon dioxide (CO2) Dry powder

Unsuitable extinguishing media

For this substance/mixture no limitations of extinguishing agents are given.

Specific hazards during fire fighting

Mixture with combustible ingredients. Vapours are heavier than air and may spread along floors. Forms explosive mixtures with air on intense heating. Development of hazardous combustion gases or vapours possible in the event of fire.

Hazardous combustion products Hazardous combustion products

Carbon oxides Nitrogen oxides (NOx) Sulphur oxides Carbon oxides Nitrogen oxides (NOx) Sulphur oxides

Specific extinguishing methods

Suppress (knock down) gases/vapours/mists with a water spray jet. Prevent fire extinguishing water from contaminating surface water or the ground water system.

Special protective equipment for fire-fighters

Stay in danger area only with self-contained breathing apparatus. Prevent skin contact by keeping a safe distance or by wearing suitable protective clothing.

SECTION 6: Accidental release measures

Personal precautions, protective equipment and emergency procedures

Advice for non-emergency personnel: Do not breathe vapours, aerosols. Avoid substance contact. Ensure adequate ventilation. Evacuate the danger area, observe emergency procedures, consult an expert. Advice for emergency responders: For personal protection see section 8.

Environmental precautions

Do not let product enter drains.

Methods and materials for containment and cleaning up

Cover drains. Collect, bind, and pump off spills. Observe possible material restrictions (see sections 7 and 10). Take up carefully with liquid-absorbent material . Dispose of properly. Clean up affected area.

SECTION 7: Handling and storage

Handling

Advice on safe handling

Work under hood. Do not inhale substance/mixture. Avoid generation of vapours/aerosols.

Avoidance of contact

Strong acids Strong oxidizing agents Strong bases Aldehydes Aluminum

Storage

Further information on storage conditions

Tightly closed. Keep locked up or in an area accessible only to qualified or authorised persons.

Storage class

10, Combustible liquids

Recommended storage temperature

-20 °C

SECTION 8: Exposure controls/personal protection

control parameter

Hazard composition and occupational exposure limits

Does not contain substances with occupational exposure limits.

Engineering measures

No data available

Personal protective equipment

Respiratory protection

required when vapours/aerosols are generated.
Our recommendations on filtering respiratory protection are based on the following standards: DIN EN 143, DIN 14387 and other accompanying standards relating to the used respiratory protection system.

Recommended Filter type

Filter type ABEK
The entrepeneur has to ensure that maintenance, cleaning and testing of respiratory protective devices are carried out according to the instructions of the producer. These measures have to be properly documented.

Eye/face protection

Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU).
Safety glasses

Skin and body protection

protective clothing

Hand protection

Remarks

required

Hygiene measures

Change contaminated clothing. Preventive skin protection recommended. Wash hands after working with substance.

SECTION 9: Physical and chemical properties

Information on basic physicochemical properties

Physical statepowder
Particle characteristics Particle sizeNo data available
Oxidizing propertiesnone
Explosive propertiesNot classified as explosive.
Flow timeNo data available
Viscosity, kinematicNo data available
Viscosity, dynamicNo data available
Evaporation rateNo data available
Flash point111 °C
Boiling point/boiling rangeNo data available
Melting point/ rangeNo data available
pHNo data available
Odor ThresholdNo data available
OdorNo data available
Colorwhite
Appearanceliquid
Decomposition temperatureNo data available
Autoignition temperatureNo data available
Partition coefficient: n-octanol/waterNo data available
Water solubilitysoluble (20 °C)
DensityNo data available
Relative densityNo data available
Relative vapor densityNo data available
Vapor pressureNo data available
Lower explosion limit / Lower flammability limitNo data available
Upper explosion limit / Upper flammability limitNo data available
Burning rateNo data available
Flammability (liquids)No data available
Flammability (solid, gas)No data available

SECTION 10: Stability and reactivity

Reactivity

Forms explosive mixtures with air on intense heating. A range from approx. 15 Kelvin below the flash point is to be rated as critical.

Chemical stability

The product is chemically stable under standard ambient conditions (room temperature) .

Possibility of hazardous reactions

Violent reactions possible with

Conditions to avoid

Strong heating.

Incompatible materials

Strong acids Strong oxidizing agents Strong bases Aldehydes Aluminum

Hazardous decomposition products

In the event of fire: see section 5

SECTION 11: Toxicological information

11.1 Information on toxicological effects

Mixture Acute toxicity

Oral: No data available
Inhalation: No data available
Dermal: No data available

Skin corrosion/irritation

Classified based on available data. For more details, see section 2

Serious eye damage/eye irritation

Classified based on available data. For more details, see section 2

Respiratory or skin sensitization

Mixture may cause allergy or asthma symptoms or breathing difficulties if inhaled.

Germ cell mutagenicity

Classified based on available data. For more details, see section 2

Carcinogenicity

Classified based on available data. For more details, see section 2

Reproductive toxicity

Classified based on available data. For more details, see section 2

Specific target organ toxicity - single exposure

Classified based on available data. For more details, see section 2

Specific target organ toxicity - repeated exposure

Mixture may cause damage to organs through prolonged or repeated exposure.
- Kidney

Aspiration hazard

Classified based on available data. For more details, see section 2

11.2 Additional Information

When ingested early symptoms mimic alcohol inebriation and are followed by nau- sea, vomiting, abdominal pain, weakness, muscle tenderness, respiratory failure, convulsions, cardiovascular collapse, pulmonary edema, hypocalcemic tetany, and severe metabolic acidosis. Without treatment, death may occur in 8 to 24 hours.
Victims who survive the initial toxicity period usually develop renal failure along with brain and liver damage., Exposure to and/or consumption of alcohol may in- crease toxic effects.
Other dangerous properties can not be excluded.
Handle in accordance with good industrial hygiene and safety practice.

Components ethylene glycol

Acute toxicity

Acute toxicity estimate Oral - 500.1 mg/kg (Expert judgement)
Remarks: Classified according to Regulation (EU) 1272/2008, Annex VI (Table 3.1/3.2)
LC50 Inhalation - Rat - male and female - 6 h - > 2.5 mg/l - aerosol
Remarks: (ECHA)
LD50 Dermal - Mouse - male and female - > 3,500 mg/kg
Remarks: (ECHA)

Skin corrosion/irritation

Skin - Rabbit
Result: No skin irritation - 20 h
Remarks: (ECHA)

Serious eye damage/eye irritation

Eyes - Rabbit
Result: No eye irritation - 24 h
Remarks: (ECHA)

Respiratory or skin sensitization

Maximisation Test - Guinea pig
Result: negative (OECD Test Guideline 406)

Germ cell mutagenicity

Test Type: Ames test
Test system: Escherichia coli/Salmonella typhimurium
Result: negative
Test Type: Mutagenicity (mammal cell test): chromosome aberration.
Test system: Chinese hamster ovary cells
Result: negative
Species: Rat - male and female
Result: negative
Remarks: (ECHA)

Carcinogenicity

This product is or contains a component that is probably not carcinogenic based on its IARC, ACGIH, NTP, or EPA classification.

Reproductive toxicity

Laboratory experiments have shown teratogenic effects.
Overexposure may cause reproductive disorder(s) based on tests with la- boratory animals.

Specific target organ toxicity - single exposure

Classified based on available data. For more details, see section 2

Specific target organ toxicity - repeated exposure

Oral - May cause damage to organs through prolonged or repeated expo- sure.
- Kidney

Aspiration hazard

Classified based on available data. For more details, see section 2

ammonium sulphate Acute toxicity

LD50 Oral - Rat - male and female - 4,250 mg/kg (OECD Test Guideline 401)
Inhalation: No data available
LD50 Dermal - Rat - male and female - > 2,000 mg/kg (OECD Test Guideline 434)

Skin corrosion/irritation

Skin - Rabbit
Result: No skin irritation - 20 h
Remarks: (ECHA)

Serious eye damage/eye irritation

Eyes - Rabbit
Result: No eye irritation
Remarks: (ECHA)

Respiratory or skin sensitization

Maximisation Test - Guinea pig
Result: negative (US-EPA)

Germ cell mutagenicity

Test Type: Ames test
Test system: S. typhimurium
Result: negative
Test Type: Mutagenicity (mammal cell test): chromosome aberration.
Test system: Human lymphocytes
Result: negative
Test Type: In vitro mammalian cell gene mutation test
Test system: Chinese hamster lung cells
Result: negative
Species: Mouse - male - Bone marrow
Remarks: (ECHA)

Carcinogenicity

Classified based on available data. For more details, see section 2

Reproductive toxicity

Classified based on available data. For more details, see section 2

Specific target organ toxicity - single exposure

Classified based on available data. For more details, see section 2

Specific target organ toxicity - repeated exposure Aspiration hazard

Classified based on available data. For more details, see section 2

Luciferase (firefly luciferin)

Acute toxicity

Oral: No data available
Inhalation: No data available
Dermal: No data available

Skin corrosion/irritation

Classified based on available data. For more details, see section 2

Serious eye damage/eye irritation

Classified based on available data. For more details, see section 2

Respiratory or skin sensitization

Prolonged or repeated exposure may cause allergic reactions in certain sensi- tive individuals.

Germ cell mutagenicity

Classified based on available data. For more details, see section 2

Carcinogenicity

Classified based on available data. For more details, see section 2

Reproductive toxicity

Classified based on available data. For more details, see section 2

Specific target organ toxicity - single exposure

Classified based on available data. For more details, see section 2

Specific target organ toxicity - repeated exposure

Classified based on available data. For more details, see section 2

Aspiration hazard

Classified based on available data. For more details, see section 2

SECTION 12: Ecological information

Ecotoxicity

Components:

ethylene glycol:

Toxicity to fish

LC50 (Pimephales promelas (fathead minnow)): 72,860 mg/l End point: mortality Exposure time: 96 h Test Type: static test Method: US-EPA

Toxicity to daphnia and other aquatic invertebrates

EC50 (Daphnia magna (Water flea)): > 100 mg/l Exposure time: 48 h Test Type: static test Analytical monitoring: yes Method: OECD Test Guideline 202 GLP: yes

Toxicity to algae/aquatic plants

NOEC (Pseudokirchneriella subcapitata (green algae)): > 100 mg/l Exposure time: 72 h Test Type: static test Analytical monitoring: yes Method: OECD Test Guideline 201 GLP: yes

Toxicity to fish (Chronic toxicity)

LC50 (Menidia peninsulae (tidewater silverside)): > 1,500 mg/l End point: mortality Exposure time: 28 d Test Type: flow-through test Analytical monitoring: yes Remarks: (ECHA) The value is given in analogy to the following substances: The value is given in analogy to the following substances: triethylene glycol

Toxicity to daphnia and other aquatic invertebrates (Chronic toxicity)

NOEC (Ceriodaphnia dubia (water flea)): 8,590 mg/l End point: reproduction rate Exposure time: 7 d Test Type: semi-static test Method: US-EPA

ammonium sulphate:

Toxicity to fish

LC50 (Oncorhynchus mykiss (rainbow trout)): 53 mg/l End point: mortality Exposure time: 96 h Analytical monitoring: yes Remarks: (ECHA)

Toxicity to daphnia and other aquatic invertebrates

EC50 (Ceriodaphnia (water flea)): 121.7 mg/l End point: Immobilization Exposure time: 48 h Test Type: static test Analytical monitoring: yes Method: US-EPA

Toxicity to algae/aquatic plants

ErC50 (Chlorella vulgaris (Fresh water algae)): 2,700 mg/l Exposure time: 18 Days Test Type: static test Remarks: (ECHA)

Toxicity to fish (Chronic toxicity)

EC10 (Lepomis macrochirus): 5.29 mg/l Exposure time: 30 d Test Type: flow-through test Analytical monitoring: yes Remarks: (ECHA)

Toxicity to daphnia and other aquatic invertebrates (Chronic toxicity)

EC10 (Daphnia (water flea)): 3.12 mg/l End point: reproduction rate Exposure time: 70 d Test Type: semi-static test Analytical monitoring: yes Method: US-EPA

Toxicity to microorganisms

EC50 (activated sludge): 1,618 mg/l Exposure time: 30 min Test Type: static test Method: OECD Test Guideline 209

Luciferase (firefly luciferin):

Toxicity to fish

Remarks: No data available

Persistence and degradability

Components:

ethylene glycol:

Biodegradability

aerobic Inoculum: activated sludge, non-adapted Concentration: 53 mg/l Result: Readily biodegradable. Biodegradation: 90 - 100 % Exposure time: 10 d Method: OECD Test Guideline 301A GLP: yes

Biochemical Oxygen Demand (BOD)

780 mg/g Incubation time: 5 d Remarks: (IUCLID)

Chemical Oxygen Demand (COD)

1,190 mg/g Remarks: (IUCLID)

ThOD

1,290 mg/g Remarks: (IUCLID)

BOD/ThOD

60 % Remarks: (IUCLID)

ammonium sulphate:

Biodegradability

Remarks: The methods for determining biodegradability are not applicable to inorganic substances.

Luciferase (firefly luciferin):

Biodegradability

Remarks: No data available

Bioaccumulative potential

Components:

ethylene glycol:

Bioaccumulation

Remarks: Does not bioaccumulate.

Partition coefficient: noctanol/water

log Pow: -1.36 Remarks: Bioaccumulation is not expected.

ammonium sulphate:

Partition coefficient: noctanol/water

Remarks: Not applicable for inorganic substances

Luciferase (firefly luciferin):

Bioaccumulation

Remarks: No data available

Mobility in soil

Components:

Luciferase (firefly luciferin):

Stability in soil

Remarks: No data available

Other adverse effects

Components:

ethylene glycol:

Results of PBT and vPvB assessment

Substance does not meet the criteria for PBT or vPvB according to Regulation (EC) No 1907/2006, Annex XIII.

ammonium sulphate:

Results of PBT and vPvB assessment

Substance does not meet the criteria for PBT or vPvB according to Regulation (EC) No 1907/2006, Annex XIII.

Additional ecological information

Biological effects: Fertilising effect possible. Discharge into the environment must be avoided.

Luciferase (firefly luciferin):

Additional ecological information

No data available

SECTION 13: Disposal considerations

Disposal methods

Waste from residues

Offer surplus and non-recyclable solutions to a licensed disposal company.

SECTION 14: Transport information

International Regulations

IATA-DGR

UN/ID No. : Not applicable
Proper shipping name : Not applicable
Class : Not applicable
Subsidiary risk : Not applicable
Packing group : Not applicable
Labels : Not applicable
Packing instruction (cargo aircraft) : Not applicable
Packing instruction (passenger aircraft) : Not applicable

IMDG-Code

UN number : Not applicable
Proper shipping name : Not applicable
Class : Not applicable
Subsidiary risk : Not applicable
Packing group : Not applicable
Labels : Not applicable
EmS Code : Not applicable
Marine pollutant : no

Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code

Not applicable for product as supplied.

National Regulations

JT/T 617

UN number : Not applicable
Proper shipping name : Not applicable
Class : Not applicable
Subsidiary risk : Not applicable
Packing group : Not applicable
Labels : Not applicable
Environmentally hazardous : no

Special precautions for user

Remarks : Not classified as dangerous in the meaning of transport regulations.

SECTION 15: Regulatory information

Catalogue of Hazardous Chemicals : This product is not listed in the catalogue of hazardous chemicals, but it meets the definition of hazardous chemicals and its principles of determination.
Downstream users need to comply with the conditions of safe use of the chemical, understand the environmental and health hazard and risk management measures identified on the SDS as well as the local/national regulations concerning the chemical.

National regulatory information

Law on the Prevention and Control of Occupational Diseases

Regulations on Safety Management of Hazardous Chemicals

Identification of Major Hazard Installations for Hazardous Chemicals (GB 18218)

Not listed

Hazardous Chemicals for Priority Management

Not listed under SAWS

Regulations on Labour Protection in Workplaces where Toxic Substances are Used

Catalogue of Highly Toxic Chemicals

Not listed

Regulation of Environmental Management on the First Import of Chemicals and the Import and Export of Toxic Chemicals

China Severely Restricted Toxic Chemicals for Import and Export

Not listed

Measures on the Environmental Administration of New Chemical Substances Registration

Registration/Notification number

B1A222225595

Regulation on the Administration of Precursor Chemicals

Catalogue and Classification of Precursor Chemicals

Not listed

SECTION 16: Other information

Full text of other abbreviations

ACGIH

USA. ACGIH Threshold Limit Values (TLV)

GBZ 2.1-2007

Occupational exposure limits for hazardous agents in the workplace - Chemical hazardous agents.

ACGIH / TWA

8-hour, time-weighted average

ACGIH / STEL

Short-term exposure limit

GBZ 2.1-2007 / PC-TWA

Permissible concentration - time weighted average

GBZ 2.1-2007 / PC-STEL AIIC - Australian Invent Transport by Land of Bra bw - Body weight; CMR Standard of the German List (Canada); ECx - Conc associated with x% respo Chemical Substances (Jap response; ERG - Emerge GLP - Good Laboratory P cer; IATA - International Construction and Equipm Half maximal inhibitory c tion; IECSC - Inventory o tional Maritime Dangerou Industrial Safety and H Standardisation; KECI - K tration to 50 % of a test (Median Lethal Dose); MA lution from Ships; MERC of Dangerous Goods; n.o. - No Observed (Adverse) fect Level; NOELR - No Norm; NTP - National Toxi icals; OECD - Organisatio fice of Chemical Safety a and Toxic substance; PIC stances; (Q)SAR - (Quant (EC) No 1907/2006 of th Registration, Evaluation, Accelerating Decompositi Chemical Substance Inve Thailand Existing Chemica States); UN - United Nat Transport of Dangerous WHMIS - Workplace Hazar

Permissible concentration - short term exposure limit ry of Industrial Chemicals
ANTT - National Agency for il
ASTM - American Society for the Testing of Materials
- Carcinogen, Mutagen or Reproductive Toxicant
DIN nstitute for Standardisation
DSL - Domestic Substances ntration associated with x% response
ELx - Loading rate se
EmS - Emergency Schedule
ENCS - Existing and New n)
ErCx - Concentration associated with x% growth rate cy Response Guide
GHS - Globally Harmonised System
actice
IARC - International Agency for Research on Canir Transport Association
IBC - International Code for the nt of Ships carrying Dangerous Chemicals in Bulk
IC50 ncentration
ICAO - International Civil Aviation Organiza- Existing Chemical Substances in China
IMDG - Interna- Goods
IMO - International Maritime Organisation
ISHL alth Law (Japan)
ISO - International Organisation for rea Existing Chemicals Inventory
LC50 - Lethal Concenopulation
LD50 - Lethal Dose to 50% of a test population POL - International Convention for the Prevention of Pol- SUR - The Agreement for the Facilitation of the Transport . - Not Otherwise Specified
Nch - Chilean Norm
NO(A)EC ffect Concentration
NO(A)EL - No Observed (Adverse) Efbservable Effect Loading Rate
NOM - Official Mexican ology Program
NZIoC - New Zealand Inventory of Chemfor Economic Co-operation and Development
OPPTS - Of- d Pollution Prevention
PBT - Persistent, Bioaccumulative S - Philippines Inventory of Chemicals and Chemical Subtative) Structure Activity Relationship
REACH - Regulation European Parliament and of the Council concerning the uthorisation and Restriction of Chemicals
SADT - Selfn Temperature
SDS - Safety Data Sheet
TCSI - Taiwan tory
TDG - Transportation of Dangerous Goods
TECI s Inventory
TSCA - Toxic Substances Control Act (United ons
UNRTDG - United Nations Recommendations on the oods
vPvB - Very Persistent and Very Bioaccumulative
ous Materials Information System

Disclaimer:

The information in this MSDS is only applicable to the specified product, unless otherwise specified, it is not applicable to the mixture of this product and other substances. This MSDS only provides information on the safety of the product for those who have received the appropriate professional training for the user of the product. Users of this MSDS must make independent judgments on the applicability of this SDS. The authors of this MSDS will not be held responsible for any harm caused by the use of this MSDS.