SBA, Soybean agglutinin

SBA, Soybean agglutinin Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

General Western Blot Protocol:

• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

1. Load samples at 500 ng of glycoprotein per lane
2. Run 4-20% Bis-Tris SDS page gel
3. Transfer gel to a PVDF membrane
4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
7. Detect using chemiluminescent substrate (CPS1-120)

Biochem/physiol Actions

SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.

SBA, Soybean agglutinin Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte

SBA, Soybean agglutinin Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Firmenname	Telefon	E-Mail	Land	Produktkatalog	Edge Rate
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80

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• Lectin from Glycine max (soybean)
• SBA, Soybean agglutinin