EST | 88321-09-9

EST Properties

Melting point	126.2°C
Boiling point	477.88°C (rough estimate)
Density	1.0657 (rough estimate)
refractive index	1.5800 (estimate)
storage temp.	-20°C
solubility	Soluble in DMSO, DMF or ethanol
pka	13.32±0.40(Predicted)
form	White solid
color	Fine needles from EtOH
Stability	Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month.
FDA UNII	L5W337AOUR

SAFETY

Risk and Safety Statements

Symbol(GHS)

GHS07

Signal word

Warning

Hazard statements

H315-H319-H335

Precautionary statements

P261-P280a-P304+P340-P305+P351+P338-P405-P501a

Hazard Codes

Xi

Risk Statements

36/37/38

Safety Statements

26-36-24/25

RIDADR

3077

WGK Germany

2

RTECS

RR0404300

HS Code

29242990

Toxicity

cyt-ham:lng 200 mg/L IYKEDH 17,815,86

NFPA 704

	0
2		0

EST price More Price(46)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	E8640	E-64d protease inhibitor	88321-09-9	250μg	$144	2024-03-01	Buy
Sigma-Aldrich	E8640	E-64d protease inhibitor	88321-09-9	1mg	$385	2024-03-01	Buy
Sigma-Aldrich	C1370	COT (30-397), active, GST tagged human PRECISIO? Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution	88321-09-9	10μg	$524	2024-03-01	Buy
Sigma-Aldrich	330005	EST A cell-permeable, irreversible inhibitor of cysteine proteases.	88321-09-9	1mg	$187	2024-03-01	Buy
TCI Chemical	E1337	E-64d [for Biochemical Research]	88321-09-9	5MG	$101	2024-03-01	Buy

Product number	Packaging	Price	Buy
E8640	250μg	$144	Buy
E8640	1mg	$385	Buy
C1370	10μg	$524	Buy
330005	1mg	$187	Buy
E1337	5MG	$101	Buy

EST Chemical Properties,Uses,Production

Description

E-64d is an irreversible, membrane-permeable inhibitor of lysosomal and cytosolic cysteine proteases and has diverse biological activities. It is a synthetic analog of E-64 and prodrug form of E-64c that inhibits calpain and the cysteine proteases cathepsins F, -K, -B, -H, and -L. E-64d (20-200 μM) induces cell cycle arrest at the G₂/M phase in A431 human epidermoid carcinoma cells. It inhibits protease-resistant prion protein accumulation in scrapie-infected neuroblastoma cells with an IC₅₀ value of 0.5 μM. E-46d also inhibits entry of vesicular stomatitis virus (VSV) particles pseudotyped with severe acute respiratory syndrome coronavirus (SARS-CoV) or SARS-CoV-2 spike glycoprotein into Vero cells, an effect that is reduced by expression of the serine protease TMPRSS2.

Uses

E-64d has been used as a lysosomal inhibitor in human acute promyelocytic leukemia NB4 cells and Huh-7?cells. It has also been used as a protease inhibitor in free calcium physiological tyrode solution for perfusion into the isolated heart samples.

Uses

E-64d is an inhibitor of cathepsins B and L as well as a potential inhibitor of calpain. E-64d has been shown to inhibit lysosomal proteases. E-64d has been used in combination with Prepstatin A to interfere with autolysosomal digestion. E-64d displays neurovascular and neuronal protective effects after focal cerebral ischemia in rats.

Definition

ChEBI: An L-leucine derivative that is the amide obtained by formal condensation of the carboxy group of (2S,3S)-3-(ethoxycarbonyl)oxirane-2-carboxylic acid with the amino group of N-(3-methylb tyl)-L-leucinamide.

Biochem/physiol Actions

E-64d is an epoxysuccinyl peptide and an inhibitor of cysteine protease cathepsin B, calpains 1 and 2. E-64d by its cathepsin B protease inhibition functionality, may serve as a potential drug for treating traumatic brain injury (TBI) and Alzheimer′s disease (AD). It inhibits gametocyte surface antigen resulting in a decreased oocyst production in Plasmodium falciparum.

Safety Profile

Moderately toxic by intraperitoneal route. An experimental teratogen. Experimental reproductive effects. Mutation data reported. Whenheated to decomposition it emits toxic fumes of NOx.

storage

Store at -20°C

References

1) McGowan?et al.?(1989),?Inhibition of calpain in intact platelets by the thiol protease inhibitor E-64d; Biochem. Biophys. Res. Commun.,?158?432 2) Wilcox and Mason (1992),?Inhibition of cysteine proteinases in lysosomes and whole cells; Biochem. J.,?285?495 3) Mizushima?et al.?(2010), Methods in mammalian autophagy research; Cell,?140?313

EST Preparation Products And Raw materials

Raw materials

Preparation Products

EST Suppliers

Global( 173)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Alpha Biopharmaceuticals Co., Ltd	+86-411-39042497 +8613921981412	sales@alphabiopharm.com	China	886	58
ATK CHEMICAL COMPANY LIMITED	+undefined-21-51877795	ivan@atkchemical.com	China	32715	60
career henan chemical co	+86-0371-86658258	sales@coreychem.com	China	29914	58
Hubei xin bonus chemical co. LTD	86-13657291602	linda@hubeijusheng.com	CHINA	22968	58
Chongqing Chemdad Co., Ltd	+86-023-6139-8061 +86-86-13650506873	sales@chemdad.com	China	39916	58
Shenzhen Shengda Pharma Limited	755-85269922 +8613424394241	sales@shengdapharm.com	CHINA	310	58
Nextpeptide Inc	+86-0571-81612335 +8613336028439	sales@nextpeptide.com	China	19915	58
InvivoChem	+1-708-310-1919 +1-13798911105	sales@invivochem.cn	United States	6393	58
Hefei TNJ Chemical Industry Co.,Ltd.	0551-65418684 +8618949823763	sales@tnjchem.com	China	25363	58
Nanjing Doge Biomedical Technology Co., Ltd	+86-25-58227606 +86-15305155328	sales@dogechemical.com	China	4128	58

Supplier	Advantage
Alpha Biopharmaceuticals Co., Ltd	58
ATK CHEMICAL COMPANY LIMITED	60
career henan chemical co	58
Hubei xin bonus chemical co. LTD	58
Chongqing Chemdad Co., Ltd	58
Shenzhen Shengda Pharma Limited	58
Nextpeptide Inc	58
InvivoChem	58
Hefei TNJ Chemical Industry Co.,Ltd.	58
Nanjing Doge Biomedical Technology Co., Ltd	58

View Lastest Price from EST manufacturers

Image	Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
	2019-07-14	EST 88321-09-9	US $7.00 / KG	1KG	99%	100KG	Career Henan Chemical Co
	2019-07-10	EST 88321-09-9	US $7.00 / KG	1KG	99%	100KG	Career Henan Chemical Co

EST
88321-09-9
US $7.00 / KG
99%
Career Henan Chemical Co

EST
88321-09-9
US $7.00 / KG
99%
Career Henan Chemical Co

EST Spectrum

EST(88321-09-9)MS EST(88321-09-9)¹HNMR

88321-09-9(EST)Related Search:

GLYCIDAMIDE (S)-(+)-Methyl glycidyl ether EST (S)-(+)-4-AMINO-3-HYDROXYBUTANOIC ACID 2-Hydroxy-4-amino butanoic acid

NALPHA-9-Fluorenylmethoxycarbonyl-S-trityl-L-cysteine pentafluorophenyl est 2,2'-[[4-[(3,5-dinitro-2-thienyl)azo]phenyl]imino]bis-ethano diacetate (est (R)-(-)-3-HYDROXY-2-METHYLPROPIONIC*ACID METHYL EST Isothiocyanic acid isobutyl est n-[3-(acetylamino)phenyl]-n-(3-methoxy-3-oxopropyl)-beta-alanin methyl est

1,2-CYCLOHEXANEDICARBOXYLICACID,BIS(2,3-EPOXYPROPYL)EST. 1,2,3,4,4a,4b,5, 6,7,9,10,10a-dodecahydro-1,4a-dimethyl-7- (1-methylethyl)-, 1,2,3-propanetriyl est1-Phenanthrenecarboxylic acid 8-FLUORO-5,6-DIHYDRO-5-METHYL-6-OXO-4H-IMIDAZO[1,5-A][1,4]BENZODIAZEPINE-3-CARBOXYLIC ACID ETHYL EST ETHYL (2S)-2,3-EPOXYPROPANOATE 2(2-HYDROXYETHOXY)ACETAMIDE

ETHYL 2,3-EPOXYPROPANOATE (2-AMINO-ETHOXY)-ACETIC ACID Loxistatin acid

1of4

COT (30-397), active, GST tagged human 2-Oxiranecarboxylicacid,3-[[[(1S)-3-methyl-1-[[(3-methylbutyl)amino]carbonyl]butyl]amino]carbonyl]-,ethyl ester, (2S,3S)- E 64d (Aloxistatin) (2S,3S)-3-[[[(1S)-3-Methyl-1-[[(3-methylbutyl)amino]carbonyl]-butyl]amino]carbonyl]-2-oxiranec L-TRANS-EPOXYSUCCINYL-LEU-3-METHYLBUTYLAMIDE-ETHYL ESTER L-TRANS-EPOXYSUCCINYL(OET)-LEU-3-METHYLBUTYLAMIDE LOXASTATIN LOXISTATIN (L-3TRANS-ETHOXYCARBONYLOXIRANE-2-CARBONYL)-L-LEUCINE-(3-METHYLBUTYL)AMIDE EST E-64D (2S,3S)-3-[[[(1S)-3-Methyl-1-[[(3-Methylbutyl)aMino]carbonyl]butyl]aMino]carbonyl]-2-oxiranecarboxylic acid ethyl ester (2S,3S)-trans-Epoxysuccinyl-L-leucylaMido-3-Methylbutane ethyl ester, EST (2S,3S)-ethyl 3-(((S)-1-(isopentylaMino)-4-Methyl-1-oxopentan-2-yl)carbaMoyl)oxirane-2-carboxylate E-64-D(LOXISTATINE) Estate oxistatin E64d,E-64d E-64(Trans-Epoxysuccinyl-L-Leucylamido-(4-Guanidino)Butane) L-trans-Epoxysuccinyl(OEt)-Leu-3-methylbuty trans-epoxysuccinyl-L-leucylamido 3-methylbutane ethyl EST, (2S,3S)-trans-Epoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester (2S,3S)-trans-epoxysuccinyl-L-*leucylamido-3-meth aloxistatin (2S)-3β-[[[(S)-3-Methyl-1-[[(3-methylbutyl)amino]carbonyl]butyl]amino]carbonyl]oxirane-2α-carboxylic acid ethyl ester [2S,3S,(+)]-3β-[(S)-3-Methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl]-2α-oxiranecarboxylic acid ethyl ester E-64d, L-trans-Epoxysuccinyl(OEt)-Leu-3-MethylbutylaMide, EP453, Aloxistatin, Loxistatin ep453 ethylester,(2s-(2-alpha,3-beta(r*)))-mino)carbonyl) (2S,3S)-ETHYL-TRANS-EPOXYSUCCINYL-L-LEUCYLAMIDO-3-METHYLBUTANE (2S,3S)-TRANS-EPOXYSUCCINYL-L-LEUCYLAMIDO-3-METHYLBUTANE ETHYL ESTER 3-[[[(1S)-3-methyl-1[[(3S-methylbutyl)amino]carbonyl]butyl]amino]carbonyl]-2S-oxiranecarboxylic acid, ethyl ester EST - CAS 88321-09-9 - Calbiochem CS-1622 ALOXISTATIN;E64D;E 64D;LOXISTATIN;E-64C ETHYL ESTER;EP 453;NSC 694281;NSC694281;NSC-694281 NSC 694281 NSC694281 NSC-694281 (2S,3S)-3-[[(2S)-4-Methyl-1-[(3-methylbutyl)amino]-1-oxo-2-pentanyl]carbamoyl]-2-oxiranecarboxylic Acid Ethyl Ester Ethyl (2S,3S)-3-[[(2S)-4-Methyl-1-[(3-methylbutyl)amino]-1-oxo-2-pentanyl]carbamoyl]-2-oxiranecarboxylate Aloxistatin (Loxistatin E64d, NSC-694281) Ethyl (2S,3S)-3-(((S)-1-(isopentylamino)-4-methyl-1-oxopentan-2-yl)carbamoyl)oxirane-2-carboxylate Aloxistatin (E-64d) EST Loxistatin Aloxistatin (E64d Aloxistatin(E-64d) E64c ethyl ester) Aloxistatin, 98%, irreversible cell-permeable cysteine protease inhibitor E-64d [for Biochemical Research] 88321-09-9 C17H30N2O5 Enzymes, Inhibitors, and Substrates Enzyme Inhibitors by Enzyme Enzyme Inhibitors A to C Calpain BioChemical Biochemicals and Reagents