Eosin is most often used as a counterstain to haematoxylin in H&E (haematoxylin and eosin) staining. H&E staining is one of the most commonly used techniques in histology.
정의
ChEBI: An organic sodium salt that is 2',4',5',7'-tetrabromofluorescein in which the carboxy group and the phenolic hydroxy group have been deprotonated and the resulting charge is neutralised by two sodium ions.
제조 방법
countries called red. Fluorescent element (C.I. Acid Yellow 73, C.I. 45350) in water or ethanol solution bromine into four bromine derivatives, and then into sodium salt.
Safety Profile
Poison by intravenous
and intraperitoneal routes. Moderately toxic
by ingestion. Questionable carcinogen with
experimental tumorigenic data. When heated
to decomposition it emits very toxic fumes
of Brand NazO. See also BROMIDES.
Purification Methods
Dissolve it in the minimum volume of H2O (1g/mL), filter and add EtOH until separation of salt is complete. Filter the solid off, wash it with absolute EtOH, then Et2O and dry it first in air, then at 100o. It is used for staining blood cells and for estimating traces of Ag. [Selsted & Becker Anal Biochem 155 270 1986, El-Ghamry & Frei Anal Chem 40 1986 1968.] [Beilstein 19 III/IV 2917.] [See above]
