BROMOSULFALEIN manufacturers and suppliers

BROMOSULFALEIN suppliers

BROMOSULFALEIN

CAS:: 71-67-0

MF:: C20H11Br4NaO10S2

MW:: 818.02

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Properties

solubility: DMF: 5 mg/ml; DMSO: 5 mg/ml; Ethanol: slightly soluble; PBS (pH 7.2): 1 mg/ml

form: Powder

Water Solubility: Soluble in water.

Sensitive: Moisture Sensitive

Merck: 14,8954

Stability:: Stable. Incompatible with strong oxidizing agents. May be air or moisture sensitive.

Safety Information

Symbol(GHS): GHS08

Signal word: Danger

Hazard statements: H317-H334

Precautionary statements: P261-P272-P280-P302+P352-P333+P313-P321-P363-P501-P261-P285-P304+P341-P342+P311-P501

Hazard Codes: Xn

Risk Statements: 42/43

Safety Statements: 22-36/37

WGK Germany: 3

RTECS: SM8395000

TSCA: Yes

Use

Purify it by TLC on silica Gel G (Merck 250a particle size) in two solvent systems (BuOH/AcOH/H2O 30:7.5:12.5 v/v, and BuOH/propionic acid/H2O 30:20:7.5 v/v). When the solvent reaches a height of ~10cm, the plate is removed, dried in air and developed with NH3 vapour giving blue-coloured spots. Also, the dye can be chromatographed on MN Silica Gel with t-BuOH/H2O/n-BuOH (32:10:5 v/v) as eluent and visualised with a dilute KOH (or NaOH if the Na salt is required) spray. The product corresponding to bromosulfalein is scraped off and eluted with H2O, filtered and evaporated to dryness in a vacuum. It was dissolved in H2O and filtered through Sephadex G-25 and evaporated to dryness. [UV and IR identification: Barbier & DeVeerdt J Pharm Sci 57 819 1968, NMR: Kato et al. Chem Pharm Bull Jpn 20 581 1972, McGuire Anal Biochem 83 75 1977, Beilstein 18/9 V 461.]

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