| Chemical Properties | Back Directory | [solubility ]
Soluble in DMSO, DMF, DCM | [form ]
Solid | [color ]
Light brown to brown | [Appearance]
Dark blue solid | [ex/em]
640/664 nm (PBS buffer) | [ε(extinction coefficient)]
250000 L⋅mol−1⋅cm−1 | [Φ(quantum yield)]
0.2 |
| Hazard Information | Back Directory | [Description]
Cy5 azide is a reactive dye containing an azide group which can participate in Click-Chemistry reactions. Cyanine5 is suitable for many different methods of fluorescence detection and can be easily detected in small quantities (nM) by naked human eye. | [Chemical Properties]
Appearance: Dark blue solid
ex/em: 640/664 nm (PBS buffer)
Extinction coefficient (ε): 250000 L??mol??1??cm??1
Quantum yield (Φ): 0.2 | [Uses]
Cyanine5 azide chloride is a potent fluorescent dye. Cyanine5 azide chloride can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). (λex=646 nm, λem=662 nm)[1]. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups. | [References]
[1] Huppelschoten Y, et, al. Total Chemical Synthesis of a Functionalized GFP Nanobody. Chembiochem. 2022 Aug 3:e202200304. DOI:10.1002/cbic.202200304 | [Abs/Em Maxima]
646/662 nm | [Fluoresene quantum yield]
0.2 | [Extinction Coefficient]
250000 | [CF260]
0.03 | [CF280]
0.04 |
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