| Identification | Back Directory | [Name]
3-METHYL-2-([1-[3-(TRIMETHYLAMMONIO)PROPYL]-4(1H)-QUINOLINYLIDENE]METHYL)-1,3-BENZOTHIAZOL-3-IUM DIIODIDE | [CAS]
157199-59-2 | [Synonyms]
TO-PRO 1
TWO-PRO? 1
To-pro 1 Iodide
TWO-PRO 1 (equivalent to TO-PRO 1) *5 mM DMSO Solution* *CAS 157199-59-2*
trimethyl-[3-[4-[(3-methyl-1,3-benzothiazol-2-ylidene)methyl]quinolin-1-ium-1-yl]propyl]azanium
trimethyl-[3-[4-[(Z)-(3-methyl-1,3-benzothiazol-2-ylidene)methyl]quinolin-1-ium-1-yl]propyl]azanium
QUINOLINIUM, 4-[(3-METHYL-2(3H)-BENZOTHIAZOLYLIDENE)METHYL]-1-[3-(TRIMETHYLAMMONIO)PROPYL]-, DIIODIDE
3-METHYL-2-([1-[3-(TRIMETHYLAMMONIO)PROPYL]-4(1H)-QUINOLINYLIDENE]METHYL)-1,3-BENZOTHIAZOL-3-IUM DIIODIDE
3-METHYL-2-([1-[3-(TRIMETHYLAMMONIO)PROPYL]-4(1H)-QUINOLINYLIDENE]METHYL)-1,3-BENZOTHIAZOL-3-IUM DIIODIDE | [Molecular Formula]
C24H29I2N3S | [MDL Number]
MFCD06409927 | [MOL File]
157199-59-2.mol | [Molecular Weight]
645.38 |
| Chemical Properties | Back Directory | [Melting point ]
>250℃ | [solubility ]
Soluble in dimethyl sulfoxide | [form ]
powder | [color ]
Red-brown | [Appearance]
Green solution | [ex/em]
515/531 nm | [ε(extinction coefficient)]
62800 cm−1M−1 | [λmax]
515 nm | [Φ(quantum yield)]
0.25 | [Biological Applications]
Nucleic acid hybridization; nucleic acid assay; nucleic acid fragment sizing; nucleic acid sequencing; detecting nucleic acids,cells,Human papilloma virus (HPV),microstructures; counting embryoblasts,leukocytes; monitoring |
| Hazard Information | Back Directory | [Description]
TO-TAP-1 (Thiazole Orange Monomer, also known as TO-PRO®-1) is a green fluorescent carbocyanine monomeric dye. TO-TAP-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.
The bright fluorescence signal and low background make TO-TAP-1 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments. TO-TAP-1 is non-cytotoxic and may be used for long-term monitoring of cell viability and dead cell detection in culture. | [Chemical Properties]
Appearance: Green solution
ex/em: 515/531 nm
Extinction coefficient (ε): 62800 cm??1M??1
Quantum yield (Φ): 0.25 | [Uses]
TO-PRO 1 is a DNA binding fluorochrome, that atached to the surface of the Feraheme (FH) nanoparticle (NP), to obtain a fluorochrome-functionalized NP. TO-PRO 1 binds DNA through intercalation, and acts as a vital fluorochrome for necrotic cells[1]. | [Definition]
ChEBI: A C1 cyanine dye having benzothiazolium-2-yl and quinolinium-4-yl substituents. | [References]
[1] Hoonsung Cho, et al. Fluorochrome-functionalized nanoparticles for imaging DNA in biological systems. ACS Nano. 2013 Mar 26;7(3):2032-41. DOI:10.1021/nn305962n | [Abs/Em Maxima]
513/533 nm |
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