| Identification | More | [Name]
EPPS | [CAS]
16052-06-5 | [Synonyms]
3-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]PROPANESULFONIC ACID
3-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]PROPANESULFONIC ACID
4-(2-HYDROXYETHYL)-1-PIPERAZINEPROPANESULFONIC ACID
4-(2-HYDROXYETHYL)PIPERAZINE-1-PROPANESULFONIC ACID
EPPS
HEPPS
LABOTEST-BB LT00138110
N-(2-HYDROXYETHYL)-N'-(3-SULFOPROPYL)-PIPERAZINE
N-[2-HYDROXYETHYL]PIPERAZINE-N'-[3-PROPANESULFONIC ACID]
N-2-HYDROXYETHYLPIPERAZINE-N'-3-PROPANESULPHONIC ACID
[2-hydroxyethyl]piperazine-n’-[3-propancesulfonicacid]
4-(2-hydroxyethyl)-1-piperazinepropanesulfonicaci
aEPPS
HEPPS N-(2-Hydroxyethyl)piperazine-N-3-propanesulfonic acid
HEPPS, ULTROL Grade
HEPPS~4-(2-Hydroxyethyl)-1-piperazinepropanesulphonic acid
4-(2-hydroxyethyl)-1-piperazinepropanesulphonic acid
EPPS FREE ACID, BIOTECHNOLOGY PERFORMANC E CERTIFIED
EPPS, 99% (4-(2-HYDROXYETHYL)-1-PIPERAZI NE-PROPANESULFONIC ACID)
EPPS SIGMAULTRA | [EINECS(EC#)]
240-198-8 | [Molecular Formula]
C9H20N2O4S | [MDL Number]
MFCD00006160 | [Molecular Weight]
252.33 | [MOL File]
16052-06-5.mol |
| Chemical Properties | Back Directory | [Appearance]
white crystalline powder | [Melting point ]
237-239 °C(lit.)
| [density ]
1.2684 (rough estimate) | [refractive index ]
1.6800 (estimate) | [storage temp. ]
Store at RT. | [solubility ]
H2O: 1 M at 20 °C, clear, colorless
| [form ]
Powder/Solid | [color ]
White | [PH]
5.0-6.5 (25℃, 0.1M in H2O) | [PH Range]
7.3 - 8.7 | [pka]
8.0(at 25℃) | [Water Solubility ]
Soluble in water. 25.2 g/L (20°C) | [λmax]
λ: 260 nm Amax: 0.055
λ: 280 nm Amax: 0.045 | [BRN ]
3957385 | [Major Application]
diagnostic assay manufacturing | [InChI]
1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15) | [InChIKey]
OWXMKDGYPWMGEB-UHFFFAOYSA-N | [SMILES]
OCCN1CCN(CCCS(O)(=O)=O)CC1 | [CAS DataBase Reference]
16052-06-5(CAS DataBase Reference) | [EPA Substance Registry System]
16052-06-5(EPA Substance) | [Absorption]
≤0.1 at 260 in H2O at 1M
≤0.1 at 280 in H2O at 1M |
| Safety Data | Back Directory | [Symbol(GHS) ]
GHS07 | [Signal word ]
Warning | [Hazard statements ]
H302-H315-H319-H332-H335 | [Precautionary statements ]
P280-P305+P351+P338-P310 | [Hazard Codes ]
Xi | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S24/25:Avoid contact with skin and eyes .
S36:Wear suitable protective clothing .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . | [WGK Germany ]
3
| [RTECS ]
TM2649130
| [TSCA ]
Yes | [REACH Registrations]
Active | [HazardClass ]
IRRITANT | [HS Code ]
29335995 | [Storage Class]
11 - Combustible Solids | [Toxicity]
quail,LD50,oral,> 316mg/kg (316mg/kg),Ecotoxicology and Environmental Safety. Vol. 6, Pg. 149, 1982. |
| Hazard Information | Back Directory | [Chemical Properties]
white crystalline powder | [Uses]
EPPS is a commonly used Good?s buffer. It is often used as separator in ultrathin isoelectric focusing gels and enhances the resolution of phosphoglucomutase. It is used as a buffering agent in biology and biochemistry. | [Definition]
ChEBI: HEPPS is a N-(sulfoalkyl)piperazine and a N-(2-hydroxyethyl)piperazine. | [General Description]
HEPPS, or N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), is a zwitterionic buffering agent commonly used in biochemical and molecular biology research. Its mechanism of action is rooted in its ability to maintain a stable pH environment, which is crucial for various enzymatic reactions and protein studies. HEPPS is especially favoured for its buffering capacity in the physiological pH range, typically between pH 6.8 and 8.2, making it ideal for biological assays and cell culture experiments. This compound is extensively utilized in preparing cell culture media, where maintaining a consistent pH is essential for cell viability and growth. Additionally, HEPPS is employed in biochemical assays, such as enzyme kinetics studies, where precise pH control is necessary to accurately measure enzymatic activity. Furthermore, HEPPS has found applications in molecular biology techniques, including nucleic acid purification, PCR (polymerase chain reaction), and protein analysis by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Its compatibility with various biological systems and stability under physiological conditions make HEPPS a valuable tool in research laboratories for maintaining optimal pH conditions and ensuring reliable experimental outcomes in various biochemical and molecular biology studies.
| [Flammability and Explosibility]
Notclassified |
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