| Identification | Back Directory | [Name]
Desthiobiotin-PEG4-DBCO | [CAS]
2032788-37-5 | [Synonyms]
Desthiobiotin-PEG4-DBCO
Desthiobiotin-PEG4-CONH-DBCO | [Molecular Formula]
C39H53N5O8 | [MDL Number]
MFCD28334544 | [MOL File]
2032788-37-5.mol | [Molecular Weight]
719.88 |
| Chemical Properties | Back Directory | [Boiling point ]
1013.4±65.0 °C(Predicted) | [density ]
1.24±0.1 g/cm3(Predicted) | [form ]
Oil | [pka]
14.93±0.60(Predicted) | [color ]
Light yellow to yellow |
| Hazard Information | Back Directory | [Description]
DBCO-PEG4-Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd?= 1011?vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin. DBCO is commonly used for copper-free Click Chemistry reactions. | [Uses]
DBCO-PEG4-Desthiobiotin is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1]. DBCO-PEG4-Desthiobiotin is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups. | [IC 50]
PEGs | [References]
[1] An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562 DOI:10.1016/j.ebiom.2018.09.005 |
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