| Identification | Back Directory | [Name]
Janelia Fluor 549, free acid | [CAS]
2245946-45-4 | [Synonyms]
Janelia Fluor 549, free acid | [Molecular Formula]
C29H23F3N2O7 | [MDL Number]
MFCD34470314 | [MOL File]
2245946-45-4.mol | [Molecular Weight]
568.51 |
| Hazard Information | Back Directory | [Uses]
Janelia Fluor? 549 TFA (JF549 TFA) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm[1]. Janelia Fluor? products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. | [Biological Activity]
Cell-permeable, yellow fluorescent dye supplied as free acid. Used for the synthesis of Janelia Fluor? HaloTag? and SNAP-Tag? ligands for use in live cell imaging experiments (Grimm et al 2017) . Also suitable for flow cytometry. Janelia Fluor? 549 is 2 x brighter than TMR and Cy3in vitroand live-cell experiments.Spectrally similar dyes:Alexa Fluor? 546, Alexa Fluor? 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3 | [storage]
-20°C | [References]
[1] Jonathan B Grimm, et al. Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol Biol. 2017;1663:179-188. DOI:10.1007/978-1-4939-7265-4_15 |
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