| Identification | Back Directory | [Name]
7ALPHA-HYDROXYCHOLESTEROL-25,26,26,26,27,27,27-D7 | [CAS]
349553-94-2 | [Synonyms]
Cholesterol Impurity 37
7α-hydroxy Cholesterol-d7
cholest-5-en-3,7α-diol-d7
7alpha-hydroxycholesterol-d7
7α-Hydroxycholesterol-25?26?26?26?27?27?27-[d7]
7ALPHA-HYDROXYCHOLESTEROL-25,26,26,26,27,27,27-D7
CHOLEST-5-EN-3,7Α-DIOL-D7;7Α-HYDROXYCHOLESTEROL-D7 | [Molecular Formula]
C26H37D7O2 | [MDL Number]
MFCD01317395 | [MOL File]
349553-94-2.mol | [Molecular Weight]
395.67 |
| Hazard Information | Back Directory | [Description]
7α-hydroxy Cholesterol-d7 is intended for use as an internal standard for the quantification of 7α-hydroxy cholesterol by GC- or LC-MS. 7α-hydroxy Cholesterol is an oxysterol and a precursor in the biosynthesis of the bile acids cholic acid (CA; ) and chenodeoxycholic acid (CDCA; ). It is formed via the oxidation of cholesterol by cholesterol 7α-hydroxylase/CYP7A1 in rat liver microsomes. 7α-hydroxy Cholesterol (40 μM) increases levels of the adhesion molecules ICAM-1, VCAM-1, and E-selectin in human umbilical vein endothelial cells (HUVECs). It increases secretion of chemokine (C-C motif) ligand 2 (CCL2) and matrix metalloproteinase-9 (MMP-9) in serum-deprived THP-1 cells when used at a concentration of 5 μg/ml. 7α-hydroxy Cholesterol has been found in macrophages isolated from atherosclerotic lesions in rabbits fed a high-cholesterol diet. | [Uses]
7α-hydroxycholesterol-d7 has been used as an internal standard for cholesterol and oxysterol measurement by gas chromatography-mass spectrometry (GC/MS). | [General Description]
7α-hydroxycholesterol is an oxysterol synthesized by the oxidation of the side chain of cholesterol. It is the precursor of bile acid. | [storage]
Store at -20°C | [References]
[1] K A MITROPOULOS S B. Cholesterol 7 -hydroxylase in rat liver microsomal preparations.[J]. Biochemical Journal, 1972, 128 1: 1-9. DOI: 10.1042/bj1280001
[2] CHIANG J Y L. Bile acid metabolism and signaling in liver disease and therapy[J]. Liver Research, 2017, 1 1: Pages 3-9. DOI: 10.1016/j.livres.2017.05.001
[3] STéPHANIE LEMAIRE. Different patterns of IL-1β secretion, adhesion molecule expression and apoptosis induction in human endothelial cells treated with 7α-, 7β-hydroxycholesterol, or 7-ketocholesterol[J]. FEBS Letters, 1998, 440 3: Pages 434-439. DOI: 10.1016/s0014-5793(98)01496-3
[4] SUN-MI KIM . 7α-Hydroxycholesterol induces inflammation by enhancing production of chemokine (C–C motif) ligand 2[J]. Biochemical and biophysical research communications, 2015, 467 4: Pages 879-884. DOI: 10.1016/j.bbrc.2015.10.050
[5] L M HULTéN. Oxysterols present in atherosclerotic tissue decrease the expression of lipoprotein lipase messenger RNA in human monocyte-derived macrophages.[J]. Journal of Clinical Investigation, 1996, 97 2: 461-468. DOI: 10.1172/jci118436 |
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