| Identification | More | [Name]
P-NITROPHENYL PHOSPHATE DI(TRIS) SALT | [CAS]
68189-42-4 | [Synonyms]
4-NITROPHENYL PHOSPHATE BIS[TRIS(HYDROXYMETHYL)AMINOMETHANE] SALT
4-NITROPHENYL PHOSPHATE DITRIS
4-NITROPHENYL PHOSPHATE DI(TRIS) SALT
4-NITROPHENYLPHOSPHORIC ACID BIS[TRIS(HYDROXYMETHYL)METHYLAMINE] SALT
4-NITROPHENYLPHOSPHORIC ACID DI[TRIS(HYDROXYMETHYL)AMINOMETHANE] SALT
P-NITROPHENYL PHOSPHATE DI(TRIS) SALT
PNPP, 2TRIS
P-NPP, DITRIS SALT
PNPP-TRIS
l)-1,3-propanediol(1:2)
p-nitrophenyl dihydrogen phosphate, compound with 2-amino-2-(hydroxymethyl)propane-1,3-diol (1:2)
p-Nitrophenylphosphate bis[tris-(hydroxymethylamino)-methane] salt
P-NITROPHENYL PHOSPHATE DITRIS
4-NitrophenylphosphateBis(Tris)Salt
4-nitrophenylphosphoric acid bis[tris(hydroxymethyl)aminomethane] salt
PNP-DITRIS SALT
4-Nitrophenylphosphoric Acid Bis[tris(hydroxymethyl)aminomethane] Salt Hydrate [Substrate for Phosphatase]
4-Nitrophenyl phosphate bistris(hydroxymethyl)aminomethaneü salt, 98%
p-NITROPHENYLPHOSPHATE DITRIS SALT crystalline extrapure
4-nitrophenyl phosphate (hydroxymethyl)aminomethane salt | [EINECS(EC#)]
206-353-9 | [Molecular Formula]
C14H28N3O12P | [MDL Number]
MFCD00037009 | [Molecular Weight]
461.36 | [MOL File]
68189-42-4.mol |
| Chemical Properties | Back Directory | [storage temp. ]
2-8°C
| [solubility ]
H2O: 0.1 g/mL, clear
| [form ]
Off-white to pale yellow solid | [color ]
White to Orange to Green | [Water Solubility ]
water: soluble | [BRN ]
1578142 | [InChI]
1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2 | [InChIKey]
XXAXKCWOTRABOW-UHFFFAOYSA-N | [SMILES]
NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O | [LogP]
-2.16 at 22℃ | [CAS DataBase Reference]
68189-42-4(CAS DataBase Reference) | [EPA Substance Registry System]
Mono(p-nitrophenyl) phosphate 2-amino-2-(hydroxymethyl)-1,3-propanediol salt (1:2) (68189-42-4) |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36:Wear suitable protective clothing . | [WGK Germany ]
3
| [F ]
8-10-21 | [TSCA ]
TSCA listed | [REACH Registrations]
Active | [HS Code ]
29199000 | [Storage Class]
11 - Combustible Solids |
| Hazard Information | Back Directory | [Uses]
4-Nitrophenyl phosphate di(tris) salt has been used:
- as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
- to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
- for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)
| [Biological Activity]
p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) proceduresbecause of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm. |
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