| Identification | More | [Name]
5-Bromo-4-chloro-3-indolyl-beta-D-galactoside | [CAS]
7240-90-6 | [Synonyms]
5-BROMO-4-CHLORO-1H-INDOL-3-YL BETA-D-GALACTOPYRANOSIDE
5-BROMO-4-CHLORO-1H-INDOL-3-YL BETA-D-GALACTOSIDE-(1,5)
5-BROMO-4-CHLORO-3-INDLOLYL-B-D-GALACTOPYRANOSIDE
5-BROMO-4-CHLORO-3-INDOLYL-B B-D-GALACTOPYRANOSIDE
5-BROMO-4-CHLORO-3-INDOLYL-B-D-GALACTOPYRANOSIDE
5'-BROMO-4-CHLORO-3-INDOLYL-BETA-D-GALACTO-PYRANOSIDE
5-BROMO-4-CHLORO-3-INDOLYL-BETA-D-GALACTOPYRANOSIDE
5-BROMO-4-CHLORO-3-INDOLYL-BETA-D-GALACTOPYRANOSIDE (X-GAL)
5-BROMO-4-CHLORO-3-INDOLYL-BETA-D-GALACTOSIDASE
5-BROMO-4-CHLORO-3-INDOLYL-BETA-D-GALACTOSIDE
5-BROMO-4-CHLORO-3-INDOLYL-BETA-D-GALATOPYRANOSIDE
5-BROMO-4-CHLORO-3-INDOXYL-BETA-D-GALACTOPYRANOSIDE
5-BROMO-4-CHLORO-3-INDOYL-BETA-D-GALACTOPYRANOSIDE
5-BROMO-4-CHLORO-3-INDOYL-BETA-D-GALACTOSIDE
BCIG
READY TO USE X-GAL
X-BETA-D-GAL
X-BETA-D-GALACTOSIDE
X-BETA-GAL
X-GAL | [EINECS(EC#)]
230-640-8 | [Molecular Formula]
C14H15BrClNO6 | [MDL Number]
MFCD00005666 | [Molecular Weight]
408.63 | [MOL File]
7240-90-6.mol |
| Chemical Properties | Back Directory | [Appearance]
White Powder | [Melting point ]
230 °C | [alpha ]
-64 º (C=1, H2O/DMF (1/1)) | [Boiling point ]
673.9±55.0 °C(Predicted) | [density ]
1.5563 (rough estimate) | [refractive index ]
1.6110 (estimate) | [storage temp. ]
2-8°C
| [solubility ]
Soluble in 100 mM in DMSO. | [form ]
Powder | [pka]
12.74±0.70(Predicted) | [color ]
White | [Odor]
Odorless | [Stability:]
Stable. Incompatible with strong oxidizing agents. | [Sensitive ]
Moisture & Light Sensitive | [Usage]
A substrate for-Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm) | [Merck ]
14,10074 | [BRN ]
1402009 | [InChIKey]
OPIFSICVWOWJMJ-AEOCFKNESA-N | [CAS DataBase Reference]
7240-90-6(CAS DataBase Reference) | [Storage Precautions]
Light sensitive;Moisture sensitive;Heat sensitive |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S24/25:Avoid contact with skin and eyes .
S37/39:Wear suitable gloves and eye/face protection .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . | [WGK Germany ]
3
| [F ]
8-10-21 | [HazardClass ]
IRRITANT | [HS Code ]
29389090 |
| Hazard Information | Back Directory | [Chemical Properties]
White Powder | [Uses]
A substrate for -Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm) | [Uses]
A substrate for β-Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm). | [Uses]
In histochemical staining, in blue-white selection of bacterial colonies with lacZ gene activity during cloning experiments. | [Uses]
magenta b-galactosidase substrate | [Definition]
ChEBI: An indolyl carbohydrate that is the beta-D-galactoside of 3-hydroxy-1H-indole in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for
he presence of an enzyme, beta-galactosidase, which cleaved the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product. | [Biological Activity]
X-gal is routinely used to detect transgene activity in situ. | [storage]
+4°C |
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