| Chemical Properties | Back Directory | [storage temp. ]
Store at -20°C | [solubility ]
DMF: 20 mg/mL; DMSO: 20 mg/mL; DMSO:PBS (pH 7.2) (1:1): 0.5 mg/mL; Ethanol: 12 mg/mL | [form ]
A crystalline solid |
| Hazard Information | Back Directory | [Description]
MitoP is a phenol product produced by the reaction of H2O2 with the ratiometric mass spectrometry probe MitoB . MitoB contains a triphenylphosphonium cation component that drives its accumulation in mitochondria where its arylboronic moiety selectively reacts with H2O2 to produce MitoP.1,2 Quantifying the MitoP/MitoB ratio by LC-MS/MS reflects the mitochondrial matrix H2O2 concentration. | [Uses]
MitoP bromide is the reaction product of MitoB with H2O2. A high MitoP/MitoB ratio indicates that the mitochondria have a high average level of H2O2[1][2]. | [References]
1. Cochemé, H.M., Quin, C., McQuaker, S.J., et al. Measurement of H2O2 within living Drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix Cell Metab. 13(3),340-350(2011).
2. Cochemé, H.M., Logan, A., Prime, T.A., et al. Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila Nat. Protoc. 7(5),946-958(2012). |
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