| Identification | More | [Name]
HEPES sodium salt | [CAS]
75277-39-3 | [Synonyms]
2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID SODIUM SALT
2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID SODIUM SALT
4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID, DISODIUM SALT
4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANE-SULFONIC ACID HALF-SODIUM SALT
4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID HEMISODIUM SALT
4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID LOWSODIUM
4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID, SODIUM SALT
4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID HEMISODIUM SALT
4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID SODIUM SALT
4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID SODIUM SALT BUFFER
HEPES
(HEPES)2-NA
HEPES, HALF-SODIUM SALT
HEPES HEMISODIUM SALT
HEPES LOWSODIUM
HEPES NA
HEPES NA-SALT
HEPES, SODIUM
HEPES SODIUM SALT
HEPES SODIUM SALT BUFFER | [EINECS(EC#)]
278-169-7 | [Molecular Formula]
C8H17N2NaO4S | [MDL Number]
MFCD00036463 | [Molecular Weight]
260.29 | [MOL File]
75277-39-3.mol |
| Chemical Properties | Back Directory | [Appearance]
white powder | [Melting point ]
234 °C | [density ]
1.504[at 20℃] | [vapor pressure ]
0Pa at 25℃ | [storage temp. ]
Store at RT. | [solubility ]
H2O: 1 M at 20 °C, clear, colorless
| [form ]
dry powder
| [color ]
White | [PH]
10.0-12.0 (1M in H2O) | [PH Range]
6.8 - 8.2 | [pka]
7.5(at 25℃) | [Stability:]
Stable. Hygroscopic-protect from moisture. Incompatible with strong oxidizing agents. | [biological source]
synthetic | [Water Solubility ]
It is soluble in water. | [Sensitive ]
Hygroscopic | [InChI]
1S/C8H18N2O4S.Na.H2O/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;;/h11H,1-8H2,(H,12,13,14);;1H2/q;+1;/p-1 | [InChIKey]
ZNJHFNUEQDVFCJ-UHFFFAOYSA-M | [SMILES]
O.[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O | [CAS DataBase Reference]
75277-39-3(CAS DataBase Reference) | [EPA Substance Registry System]
75277-39-3(EPA Substance) | [Absorption]
≤0.2 at 260 in H2O at 1M
≤0.2 at 280 in H2O at 1M |
| Hazard Information | Back Directory | [Chemical Properties]
white powder | [Uses]
HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay. | [Definition]
ChEBI: Sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate is an organic sodium salt, a N-(2-hydroxyethyl)piperazine and a N-(sulfoalkyl)piperazine. It contains a 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate. | [General Description]
HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2. | [Flammability and Explosibility]
Notclassified | [Biological Activity]
Multi purpose buffer used in biological research. | [Synthesis]
The general procedure for the synthesis of N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt from N-hydroxyethylpiperazine and sodium 2-hydroxyethanesulfonate is as follows: magnetic stirring in a 70-mL fluoropolymer-lined steel autoclave equipped with internal thermocouples for monitoring the temperature of the liquid phase, a pressure gauge, a pressure-reducing valve, and an exhaust valve. First, amine feedstock, anhydrous or aqueous sodium 2-hydroxyethanesulfonate solution, and anhydrous or aqueous sodium hydroxide solution were added to the open-topped autoclave. Subsequently, the autoclave was assembled and immersed in an electrically heated oil bath. The stirred reaction mixture was rapidly heated to the specified reaction temperature with the exhaust valve closed and maintained at that temperature for the time specified in Examples 9, 10 and 13. Upon completion of the reaction, the mixture was cooled to room temperature, the reactor was opened, and the reaction product was dissolved in a sufficient amount of water to ensure complete dissolution of all solids. | [storage]
Room temperature | [References]
[1] Patent: US2006/89509, 2006, A1. Location in patent: Page/Page column 4-6
[2] Patent: US2006/89509, 2006, A1. Location in patent: Page/Page column 4-6 |
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