| Identification | Back Directory | [Name]
Hyaluronidase | [CAS]
9001-54-1 | [Synonyms]
Aidase
Hyalas
Atidase
Diffusin
Enzodase
Mucinase
Hyaluron
Hyalidase
EC 4.2.2.1
Hyaluronipase
Hyaluronidase
HYALURONIDASES
HYALURONIDASE,BP
HYALURONATE LYASE
Hyaluronidase(fromtestes)
Hyaluronidase Streptomyces hyalurolyticus
hyaluronidasefromstreptomyceshyaluro-lyticus
HYALURONATE LYASE FROM STREPTOMYCES*HYAL UROLYTICUS
HYALURONIDASE FROM STREPTOMYCES HYALURO-LYTICUS, ~0.1U/VIAL | [EINECS(EC#)]
232-614-1 | [Molecular Formula]
n.a. | [MDL Number]
MFCD00131347 | [MOL File]
9001-54-1.mol | [Molecular Weight]
342.3 |
| Chemical Properties | Back Directory | [storage temp. ]
−20°C
| [form ]
lyophilized powder
| [color ]
white to slightly brown
| [Merck ]
13,4777 | [InChI]
InChI=1S/C18H14O7/c19-13(11-2-4-15-17(6-11)25-9-23-15)7-12(18(20)21)10-1-3-14-16(5-10)24-8-22-14/h1-6,12H,7-9H2,(H,20,21) | [InChIKey]
PPEBBOHQEAQKSW-UHFFFAOYSA-N | [SMILES]
C(O)(=O)C(C1=CC=C2OCOC2=C1)CC(C1=CC=C2OCOC2=C1)=O | [EPA Substance Registry System]
Hyaluronidase(9001-54-1) |
| Hazard Information | Back Directory | [Chemical Properties]
Hyaluronidase, an enzyme extracted from mammalian testes that hydrolyzes vitreous mucopolysaccharides, is a white or slightly yellow powder that is readily soluble in water but insoluble in ethanol, acetone, or ether. It is odorless and relatively stable, exhibiting no loss of activity after continuous heating at 420°C for 60 minutes and retaining 80% of its activity after continuous heating at 1000°C for 5 minutes. It is easily inactivated in low-concentration aqueous solutions and can generally be protected with gelatin or gum arabic. Fe2+ and Cu2+ ions reversibly inhibit the enzyme. Pb2+, Hg2+, Ni2+, and other ions have no significant effect on enzyme activity. The inhibitory effects of chondroitin sulfate B (dermatan), heparan sulfate, keratan sulfate, sodium heparin, and high-concentration hyaluronic acid on hyaluronidase can be reversed in the presence of sodium chloride (0.15 mol/L) or protamine sulfate. Hyaluronidase injection is a sterile freeze-dried product of hyaluronidase and appropriate excipients. It appears as a white or off-white freeze-dried block or powder. Aqueous solutions are unstable and must be prepared immediately before use. | [Uses]
Pharmaceutic aid (diffusing agent in s.c. injections). | [Brand name]
Diffusin
(Ortho Pharmaceutical); Enzodase (Bristol-Myers
Squibb); Hyazyme (Abbott); Vitrase (Biozyme Laboratories
Ltd., UK); Wydase (Wyeth-Ayerst);Hylase. | [Purification Methods]
Hyaluronidase is purified by chromatography on DEAE-cellulose prior to use. [Distler & Jourdain J Biol Chem 248 6772 1973.] |
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