212118-77-9
212118-77-9 结构式
基本信息
中文别名
卢米奥绿色荧光含砷螺旋粘结剂/卢米奥绿色
2-[4,5-二(1,3,2-二硫杂砷戊环-2-基)-6-羟基-3-氧代-3H-氧杂蒽-9-基]苯甲酸
英文别名
FLASHFLASH-EDT2
LuMio Green
fluorescein bis-arsenide
FLUORESCEIN ARSENICAL HELIX BINDER
FLASH Fluorescein arsenical helix binder
4',5'-bis(1,3,2-dithiarsolan-2-yl)-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
4',5'-Bis(1,3,2-dithiarsolan-2-yl)-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 4',5'-bis(1,3,2-dithiarsolan-2-yl)-3',6'-dihydroxy-
所属类别
医药中间体：原料药中间体物理化学性质
储存条件Store at -20°C,protect from light
溶解度DMF: 15 mg/ml; DMSO: 20 mg/ml; DMSO:PBS (pH 7.2) (1:10): 0.09 mg/ml
形态结晶固体
颜色White to light yellow
激发峰(Ex)/发射峰(Em)λex 509 nm; λem 529 nm
InChIInChI=1S/C24H18As2O5S4/c27-17-7-5-15-21(19(17)25-32-9-10-33-25)30-22-16(6-8-18(28)20(22)26-34-11-12-35-26)24(15)14-4-2-1-3-13(14)23(29)31-24/h1-8,27-28H,9-12H2
InChIKeyAJNUQUGWNQHQDJ-UHFFFAOYSA-N
SMILESC12(C3=C(C([As]4SCCS4)=C(O)C=C3)OC3=C1C=CC(O)=C3[As]1SCCS1)C1=C(C=CC=C1)C(=O)O2
应用领域
用途一
The Fluorescein Arsenical Hairpin Binder (FlAsH) method is presented for fluorescence labeling of proteins in living cells. Labeling proteins with the membrane-permeant fluorogenic biarsenical dye fluorescein arsenical hairpin binder-ethanedithiol (FlAsH-EDT2). The membrane-permeant fluorogenic biarsenicals FlAsH-EDT2 and ReAsH-EDT2 can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin.常见问题列表
概述
荧光含砷螺旋粘结剂/卢米奥绿色 是一种蛋白质标记试剂。FlAsH-EDT2 也是一种可透过膜的荧光双砷化合物。FlAsH-EDT2 可以与 CCXXCC 基序和非特异性内源性富含半胱氨酸的蛋白质结合。FlAsH-EDT2 仅可用于标记那些以非常高水平表达的重组蛋白。