23555-00-2
23555-00-2 结构式
基本信息
赫斯特34580游离
NO34580 荧光染料
化合物HOECHST 34580
N,N-二甲基-4-(5-(4-甲基哌嗪-1-基)-1H,1'H-[2,5'-联苯并[D]咪唑]-2'-基)苯胺
HOE 34580
HOECHST 34580
2,50-bibenzimidazole, 20-[p-(dimethylamino)phenyl]-5-(4-methyl-1-piperazinyl)-
Benzenamine, N,N-dimethyl-4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]-
物理化学性质
常见问题列表
Hoechst 34580广泛用于荧光显微镜和流式细胞术中对活细胞和固定细胞中的染色体和细胞核进行染色。
| Target | Value |
|
Aβ
(Cell-free assay) | 0.86 μM |
Hoechst 34580 prefers to locate near hydrophobic regions with binding affinity with the inhibition constant IC 50 of 0.86±0.05 μM for Hoechst 34580. Hoechst 34580 is a good candidate for treating the Alzheimer’s disease by inhibiting Aβ formation. 50 μM Aβ42 solutions co-incubated with 100, 25, 12.5, 3.125, 0.78, and 0.1, 0.01 μM Hoechst 34580 at 37 °C for 70 h. Hoechst 34580 can inhibit the aggregation of Aβ42 in a dose-dependent manner. And the half-maximal concentration (IC 50 ) is examined. The IC 50 is obtained by measuring the concentration of Hoechst 34580 while maintaining the Aβ42 concentration which gave 0.86±0.05 μM for Hoechst 34580.