3326-32-7
中文名称
异硫氰酸荧光素酯
英文名称
Fluorescein isothiocyanate isomer I
CAS
3326-32-7
EINECS 编号
248-207-7
分子式
C21H11NO5S
MDL 编号
MFCD00005063
分子量
389.38
MOL 文件
3326-32-7.mol
更新日期
2025/05/14 17:29:33
3326-32-7 结构式
基本信息
中文别名
荧光素-5-异氰酸酯异硫氰酸荧光酯异构体
异硫氰酸荧光橙红
异硫氰酸荧光红
异硫氰酸荧光黄
异硫氰酸荧光素（同分异构体I）
荧光素5-异硫氰酸盐,异构体1,95%
异硫氰酸荧光素异构体1.95+%
异硫氰酸荧光素异构体1.97%
异硫氰酸荧光酯异构体/FITC
FITC,荧光素-5-异硫氰酸盐异构体I
荧光胺异硫腈酸盐
荧光素-5-异硫氰酸盐异构体I
异硫氰酸荧光素酯
5-异氰硫基荧光素
异硫氰酸荧光酯异构体I
异硫氰酸荧光素, ISOMER 1
英文别名
2-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-5-ISOTHIOCYANATO-BENZOIC ACID5-FITC
5-FLUORESCEIN ISOTHIOCYANATE
5-FLUORESCEIN ISOTHIOCYANATEISOMER
5-FLUORESCEIN ISOTHIOCYANATE, ISOMER I
5-ISOTHIOCYANATOFLUORESCEIN
FITC
FITC-CELITE(R)
FITC ISOMER
FITC 'ISOMER I'
FITC, ISOMER I
FLUORESCEIN 5(6)-ISOTHIOCYANATE
FLUORESCEIN 5-ISOTHIOCYANATE
FLUORESCEIN 5-ISOTHIOCYANATE, ISOMER 1
FLUORESCEIN-5-ISOTHIOCYANATE ISOMER I
FLUORESCEIN ISOMER I ISOTHIOCYANATE
FLUORESCEIN ISOTHIOCYANATE
FLUORESCEIN ISOTHIOCYANATE, ISOMER 1
FLUORESCEIN ISOTHIOCYANATE ISOMER I
FLUORESCEIN ISOTHIOCYANATE ISOMER I-CELITE(R)
所属类别
化学试剂：其他酯类化合物物理化学性质
外观性质黄色粉末。有吸湿性。能与各种抗体蛋白结合，结合后的抗体不丧失与一定抗原结合的特异性，并在碱性溶液中仍有强烈绿色荧光，加酸后析出沉淀，荧光消失,微溶于丙酮、乙醚和石油醚。不溶于水。熔点:>360 °C
熔点>360 °C(lit.)
熔点>360 °C(lit.)
沸点708.6±60.0 °C(Predicted)
密度1.3423 (rough estimate)
折射率1.5060 (estimate)
储存条件2-8°C
储存条件2-8°C
溶解度微溶于DMSO、乙醇、甲醇DMF和丙酮
酸度系数(pKa)2.2, 4.4, 6.7(at 25℃)
形态powder
颜色白色
酸碱指示剂变色ph值范围Weak green uorescence (6.0) to strong green uorescence (7.2)
水溶解性几乎不溶于水
敏感性湿气敏感
最大波长(λmax)490nm
BRN1407295
稳定性稳定，但对水分敏感。与强氧化剂、强碱、胺、酸不相容。
生物领域应用Analyzing sugar
chain; detecting iodide/iodate or total iodine;
detecting/imaging thiols; detecting/quantifying biotin
derivatives;16 diagnosing classical Hodgkin lymphoma
(CHL) in lymph nodes; cellulose nanocrystals for
bioimaging applications; treating neuroinflammation;
treating/diagnosing cancer; PEO-PCL-PEO triblock
copolymers for topical delivery; polymersomes in
biomedical or cosmetic applications;apoptosis assay;
as a substrate for measuring heparanase activity, human
immunodeficiency virus (HIV-1) proteinase activity,
kinase activity, phosphatase activity, β-lactamase
activity, transglutaminase (TG2) activity; implantable
drug-delivery devicess
主要应用Magnetic nanowires, immunoassays, analyzing proteins, identifying chromosomes, Salmonella mosomes, diagnosis of cancer, kidney diseases, detecting pathogens, genes, Salmonella cells, toxoplasmosis, enzyme-mediated nucleic acid cleavage, surface molecules on colorectal cancers, treating cancer, chronic lymphocytic leukemia
InChIKeyMHMNJMPURVTYEJ-UHFFFAOYSA-N
存储注意事项湿度敏感
存储注意事项湿度敏感;光敏感
安全数据
警示词危险
危险性描述H317-H334
危险品标志Xn,Xi
危险品标志Xn
危险类别码42/43-36/37/38-22
危险类别码R42/43-R36/37/38-R22
WGK Germany3
WGK Germany3
F10
TSCAYes
海关编码29329990
常见问题列表
简介
异硫氰酸荧光素酯是黄色粉末，有吸湿性。其能与各种抗体蛋白结合，结合后的抗体不丧失与一定抗原结合的特异性，并在碱性溶液中仍有强烈绿色荧光，加酸后析出沉淀，荧光消失，微溶于丙酮、乙醚和石油醚。
用途
异硫氰酸荧光素酯能和各种抗体蛋白结合，结合后的抗体不丧失与一定抗原结合的特异性，并在碱性溶液中具有强烈的黄绿色荧光。通过在荧光显微镜下观察或流式细胞仪分析可对相应抗原进行定性、定位或定量的检测。用于医学，农学和畜牧等方面，可对由细菌病毒和寄生虫等所致疾病进行快速诊断。生物活性
FITC (Fluorescein 5-isothiocyanate) 是一种用于胺标记的荧光探针。FITC 是对 pH 和 Cu2+ 敏感的荧光染料。体外研究
Annexin V-FITC/PI is employed to detect chondrocyte apoptosis. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites.