T7 RNA POLYMERASE

T7 RNA POLYMERASE Struktur
CAS-Nr.
Englisch Name:
T7 RNA POLYMERASE
Synonyma:
T7 RNA;abs60153;T7 POLYMERASE;T7 RNA POLYMERASE
CBNumber:
CB4167044
Summenformel:
Molgewicht:
0
MOL-Datei:
Mol file

T7 RNA POLYMERASE Eigenschaften

storage temp. 
-20°C
Aggregatzustand
solution
Farbe
colorless
PH
7.9 (39°F)
Biologische Quelle
Escherichia coli (BL 21/pAR 1219)
Wasserlöslichkeit
water: miscible
Specific Activity
≥20U/μL

Sicherheit

T7 RNA POLYMERASE Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

  • T7 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from a T7 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including: RNA or DNA blotting techniques
  • In situ hybridization
  • RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
  • Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.
  • Microarray target synthesis

Allgemeine Beschreibung

T7 RNA polymerase is commonly used to transcribe DNA which has been cloned into vectors which have two phage promoters in opposite orientation. RNA can be selectively synthesized from either strand of the insert DNA with different polymerases. Homogeneously labeled single-stranded RNA can be generated with this system. Transcripts can be non-radioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-32P] or [α-35S]-labeled nucleotides.

Synthesis of hybridization probes: T7 RNA polymerase allows highly efficient production of homogeneously labeled RNA. This labeled RNA may be used as hybridization probes in Southern, northern, and dot blots, as well as in situ hybridizations.
Suitable labels:Transcripts can be nonradioactively labeled with biotin-16-UTP or DIG-11-UTP. They may also be radioactively labeled to high specific activity with [α-32P]- or [α-35S]-labeled nucleotides.

T7 RNA POLYMERASE Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


T7 RNA POLYMERASE Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global( 157)Lieferanten
Firmenname Telefon E-Mail Land Produktkatalog Edge Rate
Yinjia (Shanghai) Bio-pharmaceutical Technology Co. , Ltd. 021-52992719 13512169707
product@yinjibio.com China 29 58
Perfect mRNA 4006682586 15252998975
rd@perfectmRNA.com China 336 58
Sigma-Aldrich 021-61415566 800-8193336
orderCN@merckgroup.com China 51395 80
Shanghai Kanglang Biotechnology Co., Ltd. 021-61998208 18217752821
sales@klbio.cn China 9621 58
Absin Bioscience Inc. 021-38015121 18438616290
lanwu@univ-bio.com China 24731 58
Wuhan Chemstan Biotechnology Co., Ltd. 027-65317797 15926423062
422450190@qq.com China 10308 58
Shanghai Universal Biotech Co.,Ltd 15921930842 15921930842
yh-wang@univ-bio.com China 25006 58
Jiangsu Synthgene Biotechnology Co.,Ltd. 15852554546
starre@syngenebio.com China 901 58
Wuhan Xinzhecheng Technology Co., Ltd 17720588118
2442693357@qq.com China 144 58
Sangon Biotech (Shanghai) Co.,Ltd. 400-821-026
Sales@sangon.com China 6710 58
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