Cyanine3 azide

Description

Cy3 azide is a functionalized cyanine dye containing an azide moiety. This allows for it to undergo copper-catalyzed Click Chemistry reactions with alkynes, DBCO and BCN. The cyanine3 dye is has an excitation and emission wavelength of 555/570 nm.

Chemical Properties

Appearance: Red powder
ex/em: 554/566 nm (PBS buffer)
Extinction coefficient (ε): 150000 Lmol1cm1
Quantum yield (Φ): 0.31

Uses

Cyanine3 azide chloride, an analog of Cy3 azide, is a potent green fluorescent dye. Cyanine3 azide chloride uses click chemistry coupled with Alkyne-labeled proteins. Cyanine3 azide chloride can be detected by fluorometers, imagers, and microscopes. (λex=684 nm, λem=710 nm)[1][2]>. Cyanine3 azide (chloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

References

[1] Kim SM, et, al. Phenotypic Discovery of an Antivirulence Agent against Vibrio vulnificus via Modulation of Quorum-Sensing Regulator SmcR. ACS Infect Dis. 2020 Nov 13;6(11):3076-3082. DOI:10.1021/acsinfecdis.0c00587
[2] Morimoto K, et, al. Triazine Probes Target Ascorbate Peroxidases in Plants. Plant Physiol. 2019 Aug;180(4):1848-1859. DOI:10.1104/pp.19.00481

Abs/Em Maxima

555/570 nm

Fluoresene quantum yield

0.31

Extinction Coefficient

150000

CF260

0.04

CF280

0.09

Raw materials

Preparation Products