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| | Cyanine3 azide Basic information |
| Product Name: | Cyanine3 azide | | Synonyms: | Cyanine3 azide;3H-Indolium, 2-[3-[1-[6-[(3-azidopropyl)amino]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-2H-indol-2-ylidene]-1-propen-1-yl]-1,3,3-trimethyl-, chloride (1:1) | | CAS: | 1167421-28-4 | | MF: | C33H43N6O.Cl | | MW: | 575.19 | | EINECS: | | | Product Categories: | | | Mol File: | 1167421-28-4.mol |  |
| | Cyanine3 azide Chemical Properties |
| solubility | Soluble in DMF, DMSO, DCM | | form | Solid | | color | Green to dark green | | Appearance | Red powder | | ex/em | 554/566 nm (PBS buffer) | | ε(extinction coefficient) | 150000 L⋅mol−1⋅cm−1 | | Φ(quantum yield) | 0.31 |
| | Cyanine3 azide Usage And Synthesis |
| Description | Cy3 azide is a functionalized cyanine dye containing an azide moiety. This allows for it to undergo copper-catalyzed Click Chemistry reactions with alkynes, DBCO and BCN. The cyanine3 dye is has an excitation and emission wavelength of 555/570 nm. | | Chemical Properties | Appearance: Red powder
ex/em: 554/566 nm (PBS buffer)
Extinction coefficient (ε): 150000 Lmol1cm1
Quantum yield (Φ): 0.31 | | Uses | Cyanine3 azide chloride, an analog of Cy3 azide, is a potent green fluorescent dye. Cyanine3 azide chloride uses click chemistry coupled with Alkyne-labeled proteins. Cyanine3 azide chloride can be detected by fluorometers, imagers, and microscopes. (λex=684 nm, λem=710 nm)[1][2]>. Cyanine3 azide (chloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups. | | References | [1] Kim SM, et, al. Phenotypic Discovery of an Antivirulence Agent against Vibrio vulnificus via Modulation of Quorum-Sensing Regulator SmcR. ACS Infect Dis. 2020 Nov 13;6(11):3076-3082. DOI:10.1021/acsinfecdis.0c00587
[2] Morimoto K, et, al. Triazine Probes Target Ascorbate Peroxidases in Plants. Plant Physiol. 2019 Aug;180(4):1848-1859. DOI:10.1104/pp.19.00481 | | Abs/Em Maxima | 555/570 nm | | Fluoresene quantum yield | 0.31 | | Extinction Coefficient | 150000 | | CF260 | 0.04 | | CF280 | 0.09 |
| | Cyanine3 azide Preparation Products And Raw materials |
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