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5'(6)-FAM

5'(6)-FAM Structure
5'(6)-FAM structure
CAS No.
204697-37-0
Chemical Name:
5'(6)-FAM
Synonyms
5'-Fluorescein CE Phosphoramidite (6-FAM);5'(6)-FAM;5'-(6-FAM) CEP;5'-FAM Amidite;5'-Fluorescein CEP;FAM-Phosphoramidite;6-FAM-phosphoramidite;6-Fluorescein amidite;5'-6-FAM-PhosphoraMidite;6-FAM CE Phosphoramidite
CBNumber:
CB1726477
Molecular Formula:
C46H58N3O10P
Molecular Weight:
843.94
MDL Number:
MFCD01940925
MOL File:
204697-37-0.mol
MSDS File:
SDS
Last updated:2026-01-13 11:13:55
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5'(6)-FAM Properties

Boiling point 850.6±65.0 °C(Predicted)
storage temp. -20°C
solubility acetonitrile: soluble 0.1M, clear, colorless to faintly yellow (liquid)
pka 14.56±0.20(Predicted)
color solid white to off-white
Appearance off-white solid
InChIKey MGPYJVWEJNTXLC-UHFFFAOYSA-N
SMILES C12(C3=C(C=C(OC(=O)C(C)(C)C)C=C3)OC3=C1C=CC(OC(=O)C(C)(C)C)=C3)C1=C(C=CC(C(=O)NCCCCCCOP(N(C(C)C)C(C)C)OCCC#N)=C1)C(=O)O2
UNSPSC Code 41116105
NACRES NA.21

SAFETY

Risk and Safety Statements

WGK Germany  3
Storage Class 11 - Combustible Solids

5'(6)-FAM price More Price(20)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich M041130 6-Fluorescein Phosphoramidite configured for ABI 204697-37-0 0.1g $143 2026-04-30 Buy
Sigma-Aldrich M041130 6-Fluorescein Phosphoramidite configured for ABI 204697-37-0 0.25g $602 2026-04-30 Buy
Biosynth FF05221 5'-(6-Fluorescein) phosphoramidite 204697-37-0 0.5g $900 2026-06-04 Buy
Biosynth FF05221 5'-(6-Fluorescein) phosphoramidite 204697-37-0 0.25g $900 2026-06-04 Buy
Biosynth FF05221 5'-(6-Fluorescein) phosphoramidite 204697-37-0 0.1g $900 2026-06-04 Buy
Product number Packaging Price Buy
M041130 0.1g $143 Buy
M041130 0.25g $602 Buy
FF05221 0.5g $900 Buy
FF05221 0.25g $900 Buy
FF05221 0.1g $900 Buy

5'(6)-FAM Chemical Properties, Uses, Production

Description

FAM (fluorescein) phosphoramidite 6-isomer is specifically designed for incorporation at the 5′-end of oligonucleotides, which is essential for many biochemical assays and applications. It is primarily utilized for labeling oligonucleotides, enhancing their visibility in various assays such as PCR, sequencing and hybridization studies. It is commonly employed in real-time PCR assays where fluorescent signals are monitored during amplification. The incorporation of FAM at the 5′-end allows for efficient monitoring of the reaction progress through fluorescence.


FAM emits fluorescence with a maximal emission wavelength around 517 nm, which is ideal for various detection methods. It can be quenched by agents like DusQ 1 or DABCYL, allowing for applications in assays that require precise control over fluorescence signals.


Internal sequence additions can be achieved using modified nucleotides such as FAM-dT phosphoramidite, allowing for flexible design of probes with multiple fluorescent tags while maintaining effective spacing. However, it is essential to include spacers between the labels to prevent self-quenching of fluorescence signals, which can occur due to close proximity.


The dye soluble in common organic solvents such as acetonitrile, DCM, DMF, DMSO. Once conjugated to biomolecules, the resulting FAM conjugates can be used in aqueous applications.

Chemical Properties

5'-Fluorescein phosphoramidate is soluble in common organic solvents such as acetonitrile, DCM, DMF, and DMSO. After conjugation with biomolecules, the resulting FAM conjugates can be used in aqueous applications.

Uses

6-Fluorescein phosphoramidite is a potent fluorescent dye. 6-Fluorescein phosphoramidite can be used to label oligonucleotides[1].

References

[1] Lietard J, et al. Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA. RSC Adv. 2022 Feb 16;12(9):5629-5637. DOI:10.1039/d2ra00534d

Abs/Em Maxima

492/517 nm

Fluoresene quantum yield

0.93

Extinction Coefficient

74000

CF260

0.22

CF280

0.17

5'(6)-FAM Preparation Products And Raw materials

Raw materials

Preparation Products

5'(6)-FAM Suppliers

Global Suppliers ( 123)
Supplier Tel Email Country ProdList Advantage
Shanghai Xuanheng Pharmaceutical Technology Co., LTD 18202133986 wangweijun21@sohu.com China 473 58
Guangdong Prochem Biotechnology Co. , Ltd. 4000707101 research@prochembio.com China 133 58
Shanghai Annengjian Biomedical Technology Co., LTD 021-68781987 13818305065 sales@energybiopharma.com China 788 58
Hongene Biotech Corporation 021-64757213 info@hongene.com China 672 61
Beijing Ouhe Technology Co., Ltd 13552068683 13522913783 2355560935@qq.com China 11777 60
Wuhu Huaren Science and Technology Co., Ltd. 5842013 18155347026 xujuan@hdpharm.com China 255 64
Shanghai Hanhong Scientific Co.,Ltd. 021-54306202 13764082696 info@hanhongsci.com China 42917 64
Nanjing Asian Chemical Co., Ltd. +86 (25) 82263158 China 262 60
Bide Pharmatech Ltd. 400-164-7117 18317119277 product02@bidepharm.com China 40000 60
Shanghai ChuangYan Chemical Technology Co., Ltd. 021-11111111 11111111111 546919421@qq.com China 3769 55
Supplier Advantage
Shanghai Xuanheng Pharmaceutical Technology Co., LTD 58
Guangdong Prochem Biotechnology Co. , Ltd. 58
Shanghai Annengjian Biomedical Technology Co., LTD 58
Hongene Biotech Corporation 61
Beijing Ouhe Technology Co., Ltd 60
Wuhu Huaren Science and Technology Co., Ltd. 64
Shanghai Hanhong Scientific Co.,Ltd. 64
Nanjing Asian Chemical Co., Ltd. 60
Bide Pharmatech Ltd. 60
Shanghai ChuangYan Chemical Technology Co., Ltd. 55

View Latest Price from 5'(6)-FAM manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
6-Fluorescein phosphoramidite pictures 2025-04-04 6-Fluorescein phosphoramidite
204697-37-0
 1kg 98% 1Ton Henan Aochuang Chemical Co.,Ltd.

204697-37-0 (5'(6)-FAM) Related Search:

6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] Propanoic acid, 2,2-dimethyl-, 1,1'-[6-[10-[bis(1-methylethyl)amino]-13-cyano-1-oxo-9,11-dioxa-2-aza-10-phosphatridec-1-yl]-2',4,4',5',7,7'-hexachloro-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl] ester 5'(6)-FAM FLUORESCEIN DIPROPIONATE N-(6-Hydroxyhexyl)-6-carboxamidofluorescein dipivalate
Fluorescein 5-isothiocyanate

1of2

5'(6)-FAM 5'-(6-FAM) CEP 6-FAM-phosphoramidite (3',6'-Dipivaloylfluoresceinyl)-6-carboxamidohexyl)-1-O-(2-cyanoethyl)-(N,N-diisopropyl)phorphoramidite 5'-Fluorescein phosphoramidite 5-Fluorescein Phosphoramidite(6-FAM) 5'-6-FAM-PhosphoraMidite [(3′,6′-dipivaloylfluoresceinyl)- 6-carboxamido-hexyl]- 6-O-(2-cyanoethyl)- (N,N-diisopropyl)- phosphoramidite 6-CarboxyFluorescein-Aminohexyl Amidite 6-Fluorescein Phosphoramidite Fluorescein Phosphoramidite FAM-Phosphoramidite 6-FAM phosphoramidite [5'-Fluorescein phosphoramidite] *CAS 204697-37-0* Propanoic acid,2,2-dimethyl-,1,1'-[6-[10-[bis(1-methylethyl)amino]-13-cyano-1-oxo-9,11-dioxa-2-aza-10-phosphatridec-1-yl]-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl]ester 6-FAM phosphoramidite [5'-Fluorescein phosphoramidite 6-Fluorescein Phosphoramidite configured for ABI FAM Phosphoramidite, 6-Isomer 6-CARBOXYFLUORESCEINAMIDE PHOSPHORAMIDITE Propanoic acid,2,2-dimethyl-,1,1'-[6-[10-[bis(1-methylethyl)... 6-(3',6'-dipivaloylfluoresceinyl-6-carboxamido)-hexyl-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite 6-Fluorescein amidite 6-((6-(((2-Cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)hexyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethylpropanoate) 5'(6)-FAM (5'-Fluorescein Phosphoramidite) 6-((6-(((2-cyanoethoxy) (diisopropylamino)phosphanyl)oxy)hexyl)carbamoyl)-3-oxo-3H-spiro [isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethyl propanoate) 5'-FAM Amidite 5'-Fluorescein CEP 6'-Fluorescein CE Phosphoramidite 6-FAM phosphoramidite [5'-Fluorescein phosphoramidite] 6-FAM CE Phosphoramidite 5'-Fluorescein Phosphoramidite 5'-Fluoresceinaminophosphate 5'-Fluorescein phosphoramid 6-Fluorescein Phosphoramidite (6-FAM) (5-(4-benzamido-2-oxopyrimidin-1(2H)-yl)-3-(bis(4-methoxyphenyl)(phenyl)methoxy)-4-methoxytetrahydrofuran-2-yl)methyl (2-cyanoethyl) diisopropylphosphoramidite 5'-Fluorescein CE Phosphoramidite (6-FAM) 204697-37-0 C46H58N3O10P Other Fluorescent Conjugates Fluorescent Conjugates Fluorescent Probes, Labels, Particles and Stains Modifiers Sigma-Proligo FLUOROPHORES FAM