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PNGASE F

PNGASE F structure

CAS No.: 83534-39-8

Chemical Name:: PNGASE F

Synonyms: PNGASE A;PNGASE F;rPNGase F;EC 3.5.1.52;N-GLYCANASE;PNGase F (MS);GLYCOPEPTIDASE;N-GLYCANASE(R);PNGase F（Fast）;N-GLYCOSIDASE A

CBNumber:: CB2114680

Molecular Formula:

Molecular Weight:: 0

MDL Number:: MFCD00131222

MOL File:: Mol file

MSDS File:: SDS

Last updated:2026-07-17 09:03:53

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PNGASE F Properties

storage temp.	2-8°C
form	buffered aqueous solution
color	Colorless to light yellow
biological source	bacterial (Flavobacterium meningosepticum)
Water Solubility	water: soluble
Specific Activity	≥500mU/mg protein
UNSPSC Code	12352204
NACRES	NA.54

SAFETY

Risk and Safety Statements

Symbol(GHS)	GHS08
Signal word	Danger
Hazard statements	H334
Precautionary statements	P261-P284-P501
PPE	Eyeshields, Gloves, type N95 (US)
Hazard Codes	Xi,N,Xn
Risk Statements	37/38-41-50/53-43-20/21/22
Safety Statements	22-24/25-36/37/39-26-61
RIDADR	UN 3316 9
WGK Germany	2
Storage Class	13 - Non Combustible Solids
Hazard Classifications	Resp. Sens. 1

PNGASE F price More Price(17)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	P7367	PNGase F from Elizabethkingia meningoseptica BioReagent, ≥95% (SDS-PAGE), for proteomics	83534-39-8	50units	$449	2026-04-30	Buy
Sigma-Aldrich	P7367	PNGase F from Elizabethkingia meningoseptica BioReagent, ≥95% (SDS-PAGE), for proteomics	83534-39-8	300Units	$1990	2026-04-30	Buy
Sigma-Aldrich	NGLYFL-RO	N-Glycosidase F recombinant?form of the gene from Flavobacterium meningosepticum		100units	$452	2026-04-30	Buy
Sigma-Aldrich	NGLYFL-RO	N-Glycosidase F recombinant?form of the gene from Flavobacterium meningosepticum		250units	$821	2026-04-30	Buy
Sigma-Aldrich	G5166	PNGase F from Elizabethkingia miricola buffered aqueous solution	83534-39-8	50units	$364	2026-04-30	Buy

Product number	Packaging	Price	Buy
P7367	50units	$449	Buy
P7367	300Units	$1990	Buy
NGLYFL-RO	100units	$452	Buy
NGLYFL-RO	250units	$821	Buy
G5166	50units	$364	Buy

PNGASE F Chemical Properties,Uses,Production

Uses

Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan.

Uses

PNGase F from Elizabethkingia meningoseptica has been used:

for de-N-glycosylation of Zika20virus E protein
to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells
to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)

Uses

Glycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal of N-linked oligosaccharide chains and converts Asn residue to Asp.

General Description

PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

PNGASE F Preparation Products And Raw materials

Raw materials

Preparation Products

PNGASE F Suppliers

Global( 129)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Wuhan Chemstan Biotechnology Co., Ltd.	027-65317797 15926423062	422450190@qq.com	China	10305	58
Wuhan Tangzhi Pharmaceutical Co., Ltd.	027-63498076 13658392159	sale@glycogene.com	China	838	58
Nanjing Dulai Biotechnology Co., Ltd.	025-84699383-8003;025-846993838003-8003 18013301590	njduly@126.com	China	3293	55
Beijing Jin Ming Biotechnology Co., Ltd.	010-60605840	psaitong@jm-bio.com	China	27626	58
3B Pharmachem (Wuhan) International Co.,Ltd.	18930552037	3bsc@sina.com	China	15813	69
Hunan Hui Bai Shi Biotechnology Co., Ltd.	0731-85526065 13308475853	ivy@hnhbsj.com	China	7236	62
Dalian Meilun Biotech Co., Ltd.	0411-62910999 13889544652	sales@meilune.com	China	4765	58
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51389	80
Shanghai YuanYe Biotechnology Co., Ltd.	15026964105	2881489226@qq.com	China	89667	60
Anhui kuer Bioengineering Co., Ltd	0551-65171243 13856024262	kuer@kuerhuaxue.com	China	2996	55

Supplier	Advantage
Wuhan Chemstan Biotechnology Co., Ltd.	58
Wuhan Tangzhi Pharmaceutical Co., Ltd.	58
Nanjing Dulai Biotechnology Co., Ltd.	55
Beijing Jin Ming Biotechnology Co., Ltd.	58
3B Pharmachem (Wuhan) International Co.,Ltd.	69
Hunan Hui Bai Shi Biotechnology Co., Ltd.	62
Dalian Meilun Biotech Co., Ltd.	58
Sigma-Aldrich	80
Shanghai YuanYe Biotechnology Co., Ltd.	60
Anhui kuer Bioengineering Co., Ltd	55

View Lastest Price from PNGASE F manufacturers

Image	Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
	2026-07-17	PNGase F 83534-39-8				10g	TargetMol Chemicals Inc.

PNGase F
83534-39-8
$0.00
TargetMol Chemicals Inc.

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O-glycoproteinase

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N-Glycosidase A, PNGase A, N-linked-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase N-GlycosidaseF, Peptide N-glycosidase, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum PNGaseF from Elizabethkingia meningoseptica PNGASE FROM ELIZABETHKINGIA (CHRYSEOBACTERIUM/FLAVOBACTERIUM) MENINGSEPTICUM, RECOMBINANT EXPRESSED IN ESCHERICHIA CO PNGASE FROM ELIZABETHKINGIA (CHRYSEOBACTERIUM/FLAVOBACTERIUM) MENINGSEPTICUM, PROTEOMICS GRADE peptide-N4-(N-acetyl--glucosaMinyl) asparagineaMidase Glycopeptidase F from Elizabethkingia meningoseptica PNGase F from Elizabethkingia miricola N-linked-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase EC 3.5.1.52 GLYCOPEPTIDASE GLYCOPEPTIDASE A GLYCOPEPTIDASE F N-LINKED-GLYCOPEPTIDE-[N-ACETYL-BETA-D-GLUCOSAMINYL]-L-ASPARAGINE AMIDOHYDROLASE N-GLYCANASE N-GLYCANASE(R) N-GLYCOSIDASE A N-GLYCOSIDASE A, ALMOND N-GLYCOSIDASE F N-GLYCOSIDASE F, CHRYSEOBACTERIUM MENINGOSEPTICUM PNGASE A PNGASE F PNGASE (F CHRYSEOBACTERIUM (FLAVOBACTERIUM) MENINGOSEPTICUM) PEPTIDE-N4-(ACETYL-BETA-GLUCOSAMINYL)-ASPARAGINE AMIDASE (PEPTIDE-N4-[N-ACETYL-BETA-GLUCOSAMINYL)] PEPTIDE N-GLYCOSIDASE PEPTIDE-N-GLYCOSIDASE F PNGASE F HIGH CONCENTRATION FROM CHRYSEO PNGASE F RECOMBINANT CHRYSEOBACTERIUM GLYCOPEPTIDASE FROM ALMONDS glycopeptidase a from almonds pngase f from elizabethkingia (chryseobacterium/flavobacterium) meningsepticum GLYCOPEPTIDASE F, RECOMBINANT, SOLUTION, FROM FLAVOBACTERIUM MENINGOSEPTICUM N-Glycanase, N-GlycosidaseF, Peptide N-glycosidase, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli N-Glycosidase F, Elizabethkingia meningosepticum Native Almonds Glycopeptidase A Native Elizabethkingia meningoseptica PNGase F PNGase F from Elizabethkingia miricola, Recombinant PNGase F from Flavobacterium meningosepticum, Recombinant Native Elizabethkingia miricola PNGase F Native Flavobacterium meningosepticum PNGase F PNGase F from Elizabethkingia meningoseptica, Recombinant peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase PNGase F from Elizabethkingia meningoseptica BioReagent, >=95% (SDS-PAGE), for proteomics PNGase F from Elizabethkingia meningoseptica lyophilized powder, recombinant, expressed in E. coli PNGase F（Fast） PNGase F from Elizabethkingia meningoseptica N-Glycosidase F, Elizabethkingia meningosepticum PNGase F from Elizabethkingia miricola N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli buffered aqueous solution, recombinant, expressed in E. coli PNGase F from Elizabethkingia meningoseptica buffered aqueous solution, recombinant, expressed in E. coli Glycopeptidase A from almonds buffered aqueous glycerol solution, >=0.05 unit/mL PNGase F from Elizabethkingia miricola buffered aqueous solution PNGaseF, wildtype, liquid, recombinant, from Elizabethkingia miricola PNGase F (Glycerol-free) (MS) PNGase F (MS)