Description
st-Ht31 is a cell-permeable stearated peptide derived from the amphipathic helix domain from A-kinase anchoring proteins (AKAPs), which binds protein kinase A (PKA).1,2 It decreases PKA membrane anchoring but not activity in a reporter assay using BHK-21 fibroblasts when used at a concentration of 50 µM.2 st-Ht31 (1 and 3 µM) reduces capacitance and hyperactivation, as well as prevents in vitro fertilization, in mouse sperm.1 It increases cholesterol export from BHK-21 cells and RAW 264.7 macrophages expressing ATP-binding cassette transporter 1 (ABCA1) when used at a concentration of 50 µM.2WARNING This product is not for human or veterinary use.
References
[1] CINTIA STIVAL. Disruption of protein kinase A localization induces acrosomal exocytosis in capacitated mouse sperm.[J]. The Journal of Biological Chemistry, 2018: 9435-9447. DOI: 10.1074/jbc.ra118.002286
[2] LORETTA MA. Ht31, a protein kinase A anchoring inhibitor, induces robust cholesterol efflux and reverses macrophage foam cell formation through ATP-binding cassette transporter A1.[J]. The Journal of Biological Chemistry, 2011: 3370-3378. DOI: 10.1074/jbc.m110.173666
