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Aniline Blue

CAS No.
28631-66-5
Chemical Name:
Aniline Blue
Synonyms
ACID BLUE 22;Water-soluble aniline blue;2R;inkbluea;CI 42755;INK BLUE;OPAL BLUE;inkbluecr;inkbluegs;inkbluers
CBNumber:
CB2292270
Molecular Formula:
C32H27N3O9S3.2Na
Molecular Weight:
737.73
MDL Number:
MFCD00036130
MOL File:
28631-66-5.mol
MSDS File:
SDS
TDS File:
TDS
Last updated:2026-05-28 00:10:57
Product description Number Pack Size Price
Aniline Blue solution B8563 250ml $71.2
ACID BLUE 22 (C.I. 42755) AldrichCPR S469904 1 unit $197
ACID BLUE 22 (C.I. 42755) AldrichCPR S469904 250MG $179
Aniline Blue W.S. HARLECO?, certified by the BSC 128-M 25g $143
Aniline Blue W/S Certified 261010 100g $355
More product size

Aniline Blue Properties

storage temp. Store at +15°C to +30°C.
form Powder/Solid
color Dark brown-purple crystal with metal luster
Water Solubility water: soluble
λmax 594-610 nm
Major Application diagnostic assay manufacturing
hematology
histology
InChI 1S/C32H27N3/c1-23-22-26(16-21-31(23)33)32(24-12-17-29(18-13-24)34-27-8-4-2-5-9-27)25-14-19-30(20-15-25)35-28-10-6-3-7-11-28/h2-22,34H,33H2,1H3
InChIKey XOSXWYQMOYSSKB-LDKJGXKFSA-L
SMILES C(=C1C=CC(=NC2C=CC(S([O-])(=O)=O)=CC=2)C=C1)(C1C=CC(NC2C=CC(S(=O)(=O)O)=CC=2)=CC=1)C1=CC(=C(N)C(S([O-])(=O)=O)=C1)C.[Na+].[Na+] |t:0,4|
FDA UNII 44D829LK4D
EPA Substance Registry System C.I. Acid Blue 22 (28631-66-5)
UNSPSC Code 41116121
NACRES NA.47

SAFETY

Risk and Safety Statements

Symbol(GHS)  Exclamation Mark (GHS07)
GHS07
Signal word  Warning
Hazard statements  H315-H319-H335
Precautionary statements  P261-P264-P271-P280-P302+P352-P305+P351+P338
target organs Respiratory system
Hazard Codes  Xi,Xn
Risk Statements  36/37/38-20/21/22
Safety Statements  37/39-26-36
WGK Germany  2
TSCA  TSCA listed
HS Code  32041200
Storage Class 11 - Combustible Solids
Hazard Classifications Eye Irrit. 2
Skin Irrit. 2
STOT SE 3

Aniline Blue price More Price(42)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich B8563 Aniline Blue solution 28631-66-5 250ml $71.2 2026-04-30 Buy
Sigma-Aldrich S469904 ACID BLUE 22 (C.I. 42755) AldrichCPR 1 unit $197 2026-04-30 Buy
Sigma-Aldrich S469904 ACID BLUE 22 (C.I. 42755) AldrichCPR 28631-66-5 250MG $179 2024-03-01 Buy
Sigma-Aldrich 128-M Aniline Blue W.S. HARLECO?, certified by the BSC 28631-66-5 25g $143 2023-01-07 Buy
Usbiological 261010 Aniline Blue W/S Certified 28631-66-5 100g $355 2026-06-03 Buy
Product number Packaging Price Buy
B8563 250ml $71.2 Buy
S469904 1 unit $197 Buy
S469904 250MG $179 Buy
128-M 25g $143 Buy
261010 100g $355 Buy

Aniline Blue Chemical Properties,Uses,Production

Chemical Properties

Aniline Blue is a dark brownish-purple crystalline powder. It is soluble and used as a biological stain in fluorescent microscopy, appearing yellow-green after excitation with violet light.
Aniline Blue

Uses

Aniline Blue is a biological stain used for staining collagen and as a fluorescent stain for glycogen. It can also be used to stain collagen fibers in tissue sections using Masson's trichrome protocol for staining multiple components. In this method, collagen stains blue.

Preparation

C.I. Solvent Blue 3 (C.I. 42775) with strong sulfuric acid three stlfonation, and translated into sodium salt.

Definition

ChEBI: Aniline Blue is an organic sodium salt resulting from the formal condensation of NSC 56820 with 2 mol eq. of sodium hydroxide. It is used as a histological stain for collagen and in Mallory's method for connective tissue.

Staining Procedures

Aqueous solution, 0.5-1.0%, alternatively dissolved in ethanol, glycerol or lactoglycerol. Glucans are positive.

Properties and Applications

blue. Soluble in cold water and hot water (blue), slightly soluble in alcohol. Meet strong sulfuric acid as red light yellow, diluted into blue, and produce blue precipitation. Its water solution and sodium hydroxide is palm red light. For wool, silk, cotton, paper, leather dyeing. Used for plastic ones coloring of casein, also used in the manufacture of ink. The salt can be used as organic pigments used in rubber color.

Aniline Blue Preparation Products And Raw materials

Raw materials

Preparation Products

Global( 271)Suppliers
Supplier Tel Email Country ProdList Advantage
Wuhan Fortuna Chemical Co., Ltd
+86-27-59207850 info@fortunachem.com China 5995 58
Henan Tianfu Chemical Co.,Ltd.
+86-0371-55170693 +86-19937530512 info@tianfuchem.com China 21585 55
career henan chemical co
+86-0371-86658258 +8613203830695 sales@coreychem.com China 29812 58
BOC Sciences
+1-631-485-4226 inquiry@bocsci.com United States 19936 58
CONIER CHEM AND PHARMA LIMITED
+8618523575427 sales@conier.com China 49979 58
Career Henan Chemica Co
+86-0371-86658258 +8613203830695 laboratory@coreychem.com China 30224 58
Hefei TNJ Chemical Industry Co.,Ltd.
+86-0551-65418671 +8618949823763 sales@tnjchem.com China 34563 58
Dideu Industries Group Limited
+86-86-15536356810 +8617392712697 1022@dideu.com China 29095 58
AFINE CHEMICALS LIMITED
+86-571-85134551 info@afinechem.com China 15079 58
Henan Alfa Chemical Co., Ltd
+8615838112936 sale03@alfachem.cn China 9804 58

View Lastest Price from Aniline Blue manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Aniline Blue pictures 2026-07-22 Aniline Blue
28631-66-5
1kg 98%min 500kg WUHAN FORTUNA CHEMICAL CO., LTD
Aniline Blue pictures 2019-07-06 Aniline Blue
28631-66-5
$1.00 1kg 95%-99% 100kg Career Henan Chemical Co
MARINE BLUE V disodium hydrogen aminomethyl[[4-[(sulphonatophenyl)amino]phenyl][4-[(sulphonatophenyl)imino]cyclohexa-2,5-dien-1-ylidene]methyl]benzenesulphonate 2R ANILINE BLUE WATER-SOLUBLE FOR MICROSCOP Y ANILINE BLUE, WATER SOLUBLE, CI 42755 Aniline Blue, sodium salt, water soluble, high purity biological stain, pure Benzenesulfonic acid, aminomethyl4-(sulfophenyl)aminophenyl4-(sulfophenyl)imino-2,5-cyclohexadien-1-ylidenemethyl-, disodium salt Acid blue 22 (C.I. 42755) ANILINE BLUE ANILINE BLUE, ALC SOLUBLE ANILINE BLUE-ORCEIN ANILINE BLUE, SODIUM SALT ANILINE BLUE (SPIRIT SOLUBLE) ANILINE BLUE, WATER SOLUBLE ANILINE BLUE WS ANILINE BLUE WS SODIUM SALT ACID BLUE 3R ACID BLUE B SPIRIT BLUE SOLVENT BLUE 3 SOLUBLE BLUE G SOLUBLE BLUE 3M, 2R SOLUBLE BLUE 8B SOLUBLE BLUE B OPAL BLUE WATER BLUE WATER BLUE 6B EXTRA P WASSER-BLAU WS acidbluesb acidbluesgr acidbluesr aminomethyl((4-((sulfophenyl)amino)phenyl)(4-((sulfophenyl)imino)-2,5-cyclohexadien-1-ylidene)methyl)-benzenesulfonicacidisodiumsalt benzenesulfonicacid,aminomethyl((4-((sulfophenyl)amino)phenyl)(4-((sulfopheny benzenesulfonicacid,aminomethyl[[4-[(sulfophenyl)amino]phenyl][4-[(sulfopheny Benzenesulfonicacid,aminomethyl[[4-[(sulfophenyl)amino]phenyl][4-[(sulfophenyl)imino]-2,5-cyclohexadien-1-ylidene]methyl]-,disodiumsalt calcocidblue2r calcocidblue3b inkbluea inkbluecr inkbluegs inkbluers mitsuisolubleblueb mitsuisolublebluebnx modrkysela22 modrvodni orientsolubleblueobc paperacidbluebc puresolubleblue1811 puresolubleblue3b solubleblue2b solubleblue2r solubleblue3b solubleblue3m solubleblue3rpa solublebluec4b solubleblued400 solublebluers solubleinkblueg