T4 DNA LIGASE
- CAS No.
- 9015-85-4
- Chemical Name:
- T4 DNA LIGASE
- Synonyms
- abs60084;DNA LIGASE;EC 6.5.1.2;EC 6.5.1.1;IUB: 6.5.1.1;T4 DNA LIGASE;DNA LIGASE T4;RNA LIGASE, T4 PAHGE;Polynucleotide Ligase;DNA LIGASE, THERMOSTABLE
- CBNumber:
- CB3279078
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MFCD00163508
- MOL File:
- Mol file
- MSDS File:
- SDS
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS08 |
|---|---|
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261-P284-P501 |
| PPE | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
| WGK Germany | 3 |
| F | 10 |
| TSCA | Yes |
| HS Code | 3504009000 |
| Storage Class | 10 - Combustible liquids |
| Hazard Classifications | Resp. Sens. 1 |
T4 DNA LIGASE price More Price(14)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | DNALIG-RO | T4 DNA Ligase recombinant form of the enzyme from T4 phage | 9015-85-4 | 100units | $103 | 2026-04-30 | Buy |
| Sigma-Aldrich | DNALIG-RO | T4 DNA Ligase recombinant form of the enzyme from T4 phage | 9015-85-4 | 500units | $335 | 2026-04-30 | Buy |
| Sigma-Aldrich | D2886 | DNA Ligase from T4-infected Escherichia coli buffered aqueous glycerol solution | 9015-85-4 | 100units | $119 | 2026-04-30 | Buy |
| Sigma-Aldrich | D2886 | DNA Ligase from T4-infected Escherichia coli buffered aqueous glycerol solution | 9015-85-4 | 500units | $343 | 2022-05-15 | Buy |
| Usbiological | 313629 | DNA ligase PurifiedbyProteinGaffinitychromatography. | 50ug | $509 | 2026-06-03 | Buy |
T4 DNA LIGASE Chemical Properties, Uses, Production
Uses
Forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. For ligation of cloning vector and restriction insert fragments
General Description
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.
Contents
T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.
Biological Activity
T4 DNA Ligase forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. The ligation reaction requires ATP as a cofactor. Ligation of blunt-ended fragments requires higher enzyme concentration and can be facilitated by using PEG in the reaction mixture. The enzyme requires a 3μ hydroxyl and 5μ phosphate for ligation. Self-ligation of vector DNA can be prevented by dephosphorylation with alkaline phosphatase. T4 ligase plays an active role in repair of DNA and RNA nicks.
T4 DNA LIGASE Preparation Products And Raw materials
Raw materials
Preparation Products
T4 DNA LIGASE Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| 3B Pharmachem (Wuhan) International Co.,Ltd. | 18930552037 | 3bsc@sina.com | China | 15813 | 69 |
| Nanjing Dulai Biotechnology Co., Ltd. | 025-84699383-8003;025-846993838003-8003 18013301590 | njduly@126.com | China | 3293 | 55 |
| Shanghai BeiZhuo Biotech Co., Ltd. | 021-61119791,13386096464 | bzswkf@foxmail.com | China | 3916 | 50 |
| Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51389 | 80 |
| Shanghai YuanYe Biotechnology Co., Ltd. | 15026964105 | 2881489226@qq.com | China | 89667 | 60 |
| Beijing OKA biological technology co., LTD | 010-62971590 18548936886 | 3462612863@qq.com | China | 10061 | 58 |
| Hefei Bomei Biotechnology Co., Ltd. | 13739298932 13856598764 | 531626407@qq.com | China | 5107 | 58 |
| Guangzhou Tomums Life Science Co., Ltd. | 020-31155029 18902330969 | sales@tomums.cn | China | 4688 | 58 |
| Career Henan Chemica Co | +86-0371-86658258 +86-13203830695 | laboratory@coreychem.com | China | 30203 | 58 |
9015-85-4 (T4 DNA LIGASE) Related Search:
1of4
