| Company Name: |
Sigma-Aldrich |
| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
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| | T4 DNA LIGASE Basic information |
| Product Name: | T4 DNA LIGASE | | Synonyms: | T4 DNA LIGASE;RNA LIGASE, T4 PAHGE;DNA LIGASE;DNA LIGASE NAD-DEPENDENT;DNA LIGASE T4;DNA LIGASE, THERMOSTABLE;EC 6.5.1.1;EC 6.5.1.2 | | CAS: | 9015-85-4 | | MF: | | | MW: | 0 | | EINECS: | 232-770-0 | | Product Categories: | Enzyme | | Mol File: | Mol File | ![T4 DNA LIGASE Structure]() |
| | T4 DNA LIGASE Chemical Properties |
| storage temp. | −20°C | | form | buffered aqueous glycerol solution | | biological source | Escherichia coli (NM 989) | | Specific Activity | 4,000U/mL |
| WGK Germany | 3 | | F | 10 | | TSCA | Yes | | HS Code | 3504009000 | | Storage Class | 10 - Combustible liquids | | Hazard Classifications | Resp. Sens. 1 |
| | T4 DNA LIGASE Usage And Synthesis |
| Uses | Forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. For ligation of cloning vector and restriction insert fragments | | General Description | T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.
Contents
T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP. | | Biological Activity | T4 DNA Ligase forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. The ligation reaction requires ATP as a cofactor. Ligation of blunt-ended fragments requires higher enzyme concentration and can be facilitated by using PEG in the reaction mixture. The enzyme requires a 3μ hydroxyl and 5μ phosphate for ligation. Self-ligation of vector DNA can be prevented by dephosphorylation with alkaline phosphatase. T4 ligase plays an active role in repair of DNA and RNA nicks. |
| | T4 DNA LIGASE Preparation Products And Raw materials |
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