Yo-Pro 3

Description

YO-TAP-3 (Oxazole Red Monomer, also known as YO-PRO^®-3) is a red fluorescent carbocyanine monomeric dye. YO-TAP-3 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits significant fluorescence enhancement upon binding to DNA.

The bright fluorescence signal and low background make YO-TAP-3 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.

Simultaneous labeling with cell-impermeant YO-TAP-3 and cell-permeable LUCS 13 or Annexin V-AF488 can be used to assess cell viability and apoptosis.

Chemical Properties

ex/em : 612/631 nm
Extinction coefficient (ε) : 100100 cm1M1
Quantum yield (Φ) : 0.16

Uses

YO-PRO-3 (YO-PRO 3) is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.

Definition

ChEBI: An unsymmetrical C3 cyanine dye having 1,3-benzoxazol-2-yl and quinolinium-4-yl substituents.

References

[1] Sultana M, et al. A review on experimental chemically modified activated carbon to enhance dye and heavy metals adsorption[J]. Cleaner engineering and technology, 2022, 6: 100382.

Abs/Em Maxima

611/629 nm