PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1
- CAS No.
- 136112-00-0
- Chemical Name:
- PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1
- Synonyms
- PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1;PHENOL:CHLOROFORM:ISOAMYLALCOHOL 49.5:49 .5:1;phenol - chloroform - isoamyl alcohol mixture;PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 USP/EP/BP;Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized;PHENOL/CHLOROFORM/ISOAMYL ALCOHOL (25:24:1), STABILZED,;PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1, FOR MOL. BIOLOGY;Phenol mixt. with 3-methyl-1-butanol and trichloromethane;PHENOL:CHLOROFORM:ISOAMYL ALC. 125:24:1, FOR MOL. BIOLOGY;Phenol:Chloroform:Isoamyl Alcohol (25:24:1) pH 8.0 for molecular biology
- CBNumber:
- CB4502825
- Molecular Formula:
- C12H19Cl3O2
- Molecular Weight:
- 301.63706
- MDL Number:
- MFCD00133763
- MOL File:
- 136112-00-0.mol
| Product description | Number | Pack Size | Price |
| Phenol – chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 25:24:1 | 77617 | 100ml | $139 |
| Phenol – chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 25:24:1 | 77617 | 500ml | $443 |
| Phenol – chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 125:24:1 | 77619 | 100ml | $119 |
| Phenol – chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 125:24:1 | 77619 | 500ml | $415 |
| Phenol – chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 49.5:49.5:1 | 77618 | 100ml | $110 |
| Density | 1.29 g/mL at 20 °C |
|---|---|
| Flash point | 79 °C |
| storage temp. | 2-8°C |
| form | Liquid |
| PH | 7.7-8.3(H2O-phase, after extraction with H2O, 1:1) |
| Water Solubility | Soluble in water. |
| λmax |
λ: 330 nm Amax: 0.2 λ: 405 nm Amax: 0.1 λ: 510 nm Amax: 0.1 |
| InChI | InChI=1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H |
| InChIKey | ZYWFEOZQIUMEGL-UHFFFAOYSA-N |
| SMILES | ClC([H])(Cl)Cl.O([H])C([H])([H])C([H])([H])C([H])(C([H])([H])[H])C([H])([H])[H].O([H])C1C([H])=C([H])C([H])=C([H])C=1[H] |
| Absorption | cut-off at 360nm |
| UNSPSC Code | 12352401 |
| NACRES | ND.02 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |     GHS05,GHS06,GHS08,GHS09 |
|---|---|
| Signal word | Danger |
| Hazard statements | H301+H311+H331-H314-H341-H351-H361d-H372-H373-H411 |
| Precautionary statements | P202-P273-P280-P303+P361+P353-P304+P340+P310-P305+P351+P338 |
| target organs | Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin |
| PPE | Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter |
| Hazard Codes | T |
| Risk Statements | 23/24/25-34-40-48/20/21/22-68-63 |
| Safety Statements | 26-36/37/39-45 |
| RIDADR | UN 2810 6.1/PG 2 |
| WGK Germany | 3 |
| F | 8-10-23 |
| HazardClass | 8 |
| HS Code | 29420000 |
| Storage Class | 6.1A - Combustible acute toxic Cat. 1 and 2 very toxic hazardous materials |
| Hazard Classifications | Acute Tox. 3 Oral Acute Tox. 4 Dermal Acute Tox. 4 Inhalation Aquatic Chronic 2 Carc. 2 Eye Dam. 1 Muta. 2 Repr. 2 Skin Corr. 1B STOT RE 1 Oral STOT RE 2 STOT SE 3 |
PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 price More Price(10)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 77617 | Phenol – chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 25:24:1 | 136112-00-0 | 100ml | $139 | 2026-04-30 | Buy |
| Sigma-Aldrich | 77617 | Phenol – chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 25:24:1 | 136112-00-0 | 500ml | $443 | 2026-04-30 | Buy |
| Sigma-Aldrich | 77619 | Phenol – chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 125:24:1 | 136112-00-0 | 100ml | $119 | 2026-04-30 | Buy |
| Sigma-Aldrich | 77619 | Phenol – chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 125:24:1 | 136112-00-0 | 500ml | $415 | 2026-04-30 | Buy |
| Sigma-Aldrich | 77618 | Phenol – chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 49.5:49.5:1 | 136112-00-0 | 100ml | $110 | 2026-04-30 | Buy |
PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 Chemical Properties,Uses,Production
Chemical Properties
clear yellow solution
Uses
Phenol:Chloroform:Isoamyl alcohol 25:24:1 is used in the extraction of DNA for the generation of DNA minisatellite fingerprints. It is also used for purifying RNA.
Biological Activity
When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
Synthesis
Examples of the application of phenol-chloroform-isoamyl alcohol mixture are as follows:
(1) For the extraction of genomic DNA of a glycoconjugate-producing bacterium suitable for whole genome sequencing, extraction buffer and lysozyme were added to a culture of glycoconjugate-producing bacterium, which was left to stand for 10 min at 37C, then proteinase K was added, and left to stand for 1 h at 65C; a mixture of phenol-chloroform-isoamyl alcohol was added, and extracted; a mixture of chloroform-isoamyl alcohol was added, and extracted; cold ethanol and sodium acetate were added, and the precipitate was washed by ethanol and dried at room temperature, and RNA was added to dissolve it. centrifugation, the precipitate was washed with ethanol and dried at room temperature, and solubilized by adding DNA extraction buffer; add RNAase, and let it stand for 10 min at 37; add PEG6000, mix well, and let it stand for 10 min at 50; add a mixed solution of phenol-chloroform-isoamyl alcohol, and extract; add a mixed solution of chloroform-isoamyl alcohol, and extract; add a solution of cold ethanol and sodium acetate, and centrifugation, the precipitate was washed with ethanol and dried at room temperature, and the precipitate was washed with ethanol. After drying at room temperature, add ddH2O or TE buffer to dissolve, which is the extracted DNA solution.
PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 Preparation Products And Raw materials
Raw materials
Preparation Products
PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Hubei Ipure Biology Co., Ltd | +8618062405514 | ada@ipurechemical.com | China | 10224 | 58 |
| HONG KONG IPURE BIOLOGY CO.,LIMITED | 86 18062405514 18062405514 | ada@ipurechemical.com | CHINA | 3453 | 58 |
| Dideu Industries Group Limited | +86-29-89586680 +86-15129568250 | 1059@dideu.com | China | 29096 | 58 |
| Alfa Chemistry | +1-5166625404; | Info@alfa-chemistry.com | United States | 20000 | 58 |
| chemmole | 400-168-9330 15013243073 | 597760450@qq.com | China | 2938 | 58 |
| Meryer (Shanghai) Chemical Technology Co., Ltd. | 4006356688 18621169109 | market03@meryer.com | China | 40202 | 62 |
| Sinopharm Chemical Reagent Co,Ltd. | 86-21-63210123 | sj_scrc@sinopharm.com | China | 9791 | 79 |
| Thermo Fisher Scientific | 800-810-5118 | cnchemical@thermofisher.com | China | 17752 | 75 |
| Codow Chemical Co.,Ltd. | -- | amy@howeipharm.com | China | 6669 | 55 |
| Chengdu Huachun Technology Co., Ltd | 400-1166-196 15982826727 | cdhxsj@163.com | China | 14571 | 60 |
View Lastest Price from PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2025-11-18 | PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 USP/EP/BP
136112-00-0
|
$1.10 | 1g | 99.9% | 100 Tons min | Dideu Industries Group Limited |
-
- PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 USP/EP/BP
136112-00-0
- $1.10
- 99.9%
- Dideu Industries Group Limited
